二氢杨梅素对巨噬细胞源性泡沫细胞胆固醇流出的影响

摘要：目的 观察二氢杨梅素 （DMY） 对鼠源巨噬细胞性泡沫细胞胆固醇流出的影响并探讨其可能机制。方法 用 50 mg/L 的氧化低密度脂蛋白 （ox-LDL） 孵育鼠源性巨噬细胞 RAW264.7 经 48 h 诱导细胞泡沫化。将泡沫细胞分为对照组 （只加 RPMI 1640 的培养基培养） 和 DMY1~4 组 （分别用 10、 20、 40 和 80 μmol/L DMY 处理）， 培养 24 h。采用 3 H]标记的胆固醇检测细胞胆固醇的流出率， 高效液相色谱测定细胞内游离胆固醇 （FC）、 胆固醇酯 （CE） 和总胆固醇 （TC） 的水平， Western blot 检测细胞中三磷酸腺苷结合盒转运体 A1 （ABCA1） 的表达。结果 与对照组比较， 20、 40 和 80 μmol/L DMY 组细胞的胆固醇流出率均显著增加， 细胞内 FC、 TC 和 CE 水平及 CE/TC 的比值均显著减少， ABCA1 的表达显著上调， 均呈剂量依赖性 （均 P < 0.05）。与对照组比较， DMY （10 μmol/L） 组细胞胆固醇流出率，细胞内 FC、 TC 和 CE 水平，ABCA1 的表达差异均无统计学意义 （均 P > 0.05）。结论 DMY 促进了鼠源巨噬细胞性泡沫细胞胆固醇流出， 其机制可能与上调泡沫细胞中 ABCA1的表达有关。 ,

Effects of dihydromyricetin on the cholesterol efflux in macrophage derived foam cells

Abstract: Objective To explore the effect of dihydromyricetin (DMY) on the cholesterol efflux in macrophage derived foam cells and analyze the possible mechanisms. Methods RAW 264.7 macrophages were incubated by oxidized low density lipoprotein (ox-LDL, 50 mg/L) for 48 h to induce foam cells. Subsequently, the foam cells were subdivided into control group (RPMI1640 media) and DMY 1-4 groups (10, 20, 40 and 80 μmol/L) and cultured for 24 h. Cholesterol efflux from foam cells was examined by 3 H] labed cholesterol. The high performance liquid chromatography assay was used to test the cellular contents of free cholesterol (FC), cholesteryl ester (CE) and total cholesterol (TC). The expression of ATP-binding cassette transporter A1 (ABCA1) was measured by Western blot assay. Results Compared with control group, cholesterol efflux was significantly increased, the content of FC, TC CE and CE/TC ratio were significantly decreased and expression of ABCA1 was significantly up-regulated in dose dependent manner in DMY (20, 40 and 80 μmol/L) groups (P < 0.05). There were no significant differences in cholesterol efflux, the content of FC, TC and CE, and expression of ABCA1 between control group and DMY (10 μmol/L) group of foam cells (P > 0.05). Conclusion DMY promotes the cholesterol efflux in the macro⁃ phage derived foam cells, which may be related with the increase of ABCA1 induced by DMY.