乌鲁木齐腹泻婴幼儿轮状病毒感染的分子特征

目的 分析乌鲁木齐腹泻婴幼儿轮状病毒(Rotavirus，RV)感染流行情况及其基因型特征。 方法 将2007年1~12月收集于乌鲁木齐市第一人民医院(儿童医院)的3 662例5岁以下门诊和住院腹泻患儿粪便标本，采用胶体金法和ELISA法检测RV，并随机选取部分RV阳性样本进行G/P基因分型。 结果 3 662例样本中检出RV阳性1 470例，检出率为40.1%。对其中142例阳性样本(约10%)进行RV PCR基因分型，其中121例婴幼儿阳性分型结果显示:G1型比例最高占36.4%，其次为G3型(20.7%)、G2型(15.7%)和G9型(13.2%)，G型混合感染占6.6%，G未能分型9例(7.4%)。P型分型以P[8]为主，占60.3%，其次为P[4](18.2%)和P[6](5.8%)，P混合感染占15.7%。最主要的G/P组合为G1P[8](28.1%，34/121)，其次是G3P[8](19.8%，24/121)、G2P[4](14.9%，18/121)和G9P[8](8.3%，10/121)，并检测到不常见组合基因型如G1P[6]、G1P[4]、G9P[4]和G9P[6]等。21例新生儿(≤28 d)阳性样本的分型结果为:G型以G9为主，占42.9%(9例)，其次是G1(4例)，G3(3例)，G2(1例)，G混合感染3例，未能分型1例。P型以P[6]为主，占47.6%(10例)，其次是P[8]及Pmixed各5例，P[4]1例。G/P基因型组合以G9P[6]为主(6例)，其次G1P[8]、G3P[8]和G1P[6]各2例，G2P[4]和G9P[8]各1例。 结论 本年度轮状病毒流行基因型复杂多样。婴幼儿RV感染以G1P[8]为主，新生儿RV感染则以G9P[6]为主。需警惕婴幼儿RV株与新生儿RV株的交叉混合感染而致新流行株的产生。 

Molecular characterization of rotavirus in children with diarrhea in Urumqi

Objective To analyze the epidemiology and genotype characteristics of group A rotavirus in children under 5years old with diarrhea in Urumqi. Methods During the year of 2007,a total of 3 662 diarrhea stool samples were collected from outpatients and children hospitalized under the age of 5 in the First People's Hospital of Urumqi. RV antigens were detected by colloidal gold assay and ELISA. About 10% RV positive samples were randomly selected for G/P genotyping. Results RV was detected in 1 470 of 3 662 samples(40. 1%) and a total of 142 positive samples were typed by PCR. Of the 121 infants and children with RV infection,G1 accounted for 36. 4%,followed by G3(20. 7%),G2(15. 7%) and G9(13. 2%),the G mixed infection was 6. 6% and 9 couldn't be typed. For P-type,P[8]was the predominant genotype with 60. 3%,followed by P[4](18. 2%),and P[6](5. 8%). P mixed was 15. 7%. The major G/P combination was G1P[8](28. 1%),next for G3P[8](19. 8%),G2P[4](14. 9%) and G9P[8](8. 3%). G1P[6],G1P[4],G9P[4] and G9P[6] were also detected as the G/P uncommon combinations. 21 neonates with RV diarrhea showed G9 was the major G genotype(9/21),followed by G1(4/21),G3(3/21) and G2(1/21). G mixed infections were3 and 1 was undetermined. P[6] ranked first with 10,P[8] and P mixed were 5,respectively. One was P[4]. G9P[6]was found to be the major G/P type in neonates. G1P[8],G3P[8] and G1P[6] were detected for 2 each and G2P[4],G9P[8] for one each in neonates. Conclusion RV genotypes were complex and diverse in 2007 in Urumqi. G1P[8]was the main G/P combination in infants and children,and G9P[6]was the main in neonates. It needs to be mindful that the mixed infection between infant RV strains and neonatal RV strains will produce new epidemic strain.