Ti-6Al-7Nb 喷砂酸蚀处理后对大鼠成骨细胞的影响

摘要 目的 纯钛（Ti）和Ti-6Al-7Nb(TC20)经机械打磨和喷砂酸蚀(SLA)两种不同方法处理后，研究其内部结构、表面能对小鼠成骨细胞的影响。方法 选取纯钛和Ti-6Al-7Nb钛片若干分为实验组和对照组，对照组直接用砂纸机械打磨，实验组用含有Al203的大颗粒石英砂和配比好的酸蚀液喷砂酸蚀两种钛片表面，将从小鼠中提取的成骨细胞接种于钛片上，使用低倍数显微镜观察经过不同处理的两种钛片表面形貌，接触角测量仪分析钛片表面亲水性，将喷砂酸蚀处理的两种钛片放入模拟体液(SBF)中7d、14d、21d后用扫描电镜(SEM)、X线衍射仪(XRD)观察样品表面沉积物形貌、物相，扫描电镜观察小鼠成骨细胞形貌，MTT测量小鼠成骨细胞增殖情况。结果 纯钛和Ti-6Al-7Nb打磨组表面呈机械划痕，喷砂酸蚀组表面拥有大量孔洞形貌，喷砂酸蚀处理后的钛片表面为亲水性结构，样品浸入模拟体液14天后Ti-6Al-7Nb表面最先观察到表面覆盖的羟基磷灰石涂层， 样品浸入模拟体液后21d,Ti和Ti-6Al-7Nb表面都观察到羟基磷灰石涂层，小鼠成骨细胞大量附着在喷砂酸蚀处理的钛片表面，经统计学分析成骨细胞在喷砂酸蚀组表面增殖能力强于光滑组（P<0.05）。结论 喷砂酸蚀处理的Ti-6Al-7Nb有利于成骨细胞的黏附, 羟基磷灰石涂层有利于促进种植体与骨组织的结合。

The effects of Ti-6Al-7Nb surface by sanding acid etch treatment on rats osteoblast

Abstract Objective study the effectiveness of internal structure and surface energy of Titanium (Ti) and Ti-6Al-7Nb (TC20) are treated by mechanical grinding and sandblasting etching on rat osteoblasts. Methods Pure titanium and Ti-6Al-7Nb were divided into experimental group and control group, control group directly mechanical polishing with sand paper, the experimental group uses large particles containing the Al203 and good ratio acid etching liquid to sandblasted and acid-etched corrosion two titanium surface, Then osteoblasts extracted from rats were seeded on titanium sheets,?low multiple microscope analyze titanium surface morphology, contact Angle measurement instrument analyze titanium surface hydrophily, the two groups by SLA were soaked into SBF for 7d，14d, 21d, then SEM and XRD observe surface sediment morphology and phase, observe different titanium surface adhesion of osteoblast cells by scanning electron microscope and analyze the proliferation of osteoblast by MTT test. Results Pure titanium and Ti-6Al-7Nb surface in mechanical grinding group showed scratches, in SLA group showed a large hole morphology and titanium surface is hydrophilic structure, samples soaked into SBF for 14d, Ti-6Al-7Nb surface firstly observed the hydroxyapatite coating, Ti and Ti-6Al-7Nb surface both observed the hydroxyapatite coating after 21d, a lot of mouse osteoblasts adhere in titanium surface with sandblasting acid corrosion processing, the proliferative ability of osteoblasts in sandblasting acid corrosion was stronger than smooth groups through the statistical analysis(P<0.05). Conclusion Ti-6Al-7Nb by SLA is advantageous to the osteoblast adhesion, hydroxyapatite coating is helpful to promote the combination of implant and bone tissue.