高效液相色谱-蒸发光散射检测法同时测定壮药愈疡散中3种主要活性成分的含量

目的：建立测定壮药愈疡散中黄芪甲苷、紫丁香苷与长梗冬青苷含量的方法。方法：采用高效液相色谱-蒸发光散射检测（HPLC-ELSD）法，色谱柱为Shim-pack CLC-ODS（150 mm×4.6 mm，5 μm），以乙腈-水为流动相，梯度洗脱，流速1 mL·min^-1，柱温20℃；检测参数为漂移管温度为90℃，雾化器温度为45℃，气体流量为1.60 L·min^-1，增益值为1.0。结果：黄芪甲苷、紫丁香苷和长梗冬青苷分别在0.46~2.76 μg（r=0.999 2）、0.70~4.20 μg（r=0.996 2）和1.06~6.36 μg（r=0.998 6）范围内，其峰面积的自然对数与进样量的自然对数呈良好的线性关系；平均加样回收率分别为95.89%、95.49%和97.66%，RSD为2.79%、3.96%和3.60%（n=6）。结论：该法简便快速、重复性好，结果准确，可作为壮药愈疡散的质量控制方法。

Simultaneous determination of three main active ingredients in Zhuang medicine Yuyangsan by HPLC-ELSD

OBJECTIVE To establish a method for content determination of astragaloside Ⅳ, syringin and pedunculoside in Zhuang medicine Yuyangsan.METHODS HPLC-ELSD method was used. Analysis was carried on Shim-pack CLC-ODS (150 mm×4.6 mm, 5 μm) column with acetonitrile-water system as mobile phase in gradient elution. The flow rate was 1 mL·min^-1, column temperature was 20℃. Drift tube temperature was 90℃, atomizing air temperature was 45℃. Gas flow rate was 1.60 L·min^-1 and gain value was 1.RESULTS Linear ranges of astragaloside Ⅳ, syringin and pedunculoside were 0.46-2.76 μg (r=0.999 2), 0.70-4.20 μg (r=0.9962) and 1.06-6.36 μg (r=0.998 6), respectively. Average recoveries were 95.89% (RSD=2.79%), 95.49% (RSD=3.96%) and 97.66% (RSD=3.60%).CONCLUSION This method is simple, rapid and steady, with good repeatability, and can be used for quality control for Zhuang medicine Yuyangsan.