NSCL/P患者中异常表达microRNA与WNT家族的关联性研究

目的:探讨非综合征性唇腭裂患者中microRNA表达谱差异及显著改变microRNA与WNT家族的关联性。方法:采用涵盖当前miRbase数据库中所有人类microRNA的miRCURY^TM探针的基因芯片对4例非综合征性唇腭裂患者的唇腭组织与4例正常新生儿脐带组织所提取的microRNA进行分析研究,并对差异性表达显著的microRNA进行生物信息学分析。结果:共筛选出254个与非综合征性唇腭裂相关的差异表达microRNA:181个在非综合征性唇腭裂组织中表达上调,73个表达下调(P＜0.05)。根据差异倍数与P值大小,选取十个表达差异较大的microRNAs进行生物信息学靶基因预测,结果显示hsa-miR-1260b, hsa-miR-205-5p, hsa-miR-24-3p, hsa-miR-27b-3p和 hsa-miR-720均靶向结合于Wnt5a、Wnt9b、Wnt10a等Wnt家族的信号分子。结论:差异表达较显著的microRNA通过与Wnt家族信号分子靶向结合参与调控唇腭裂的发育过程。

Correlation Between Abnormal MicroRNA Expression with Wnt Family in NSCL/P Patients

Objective: To investigate the miRNA expression in nonsyndromic cleft lip patients with or without cleft palate and its correlation with Wnt family in nonsyndromic cleft lip patients with or without cleft palate. Methods: Gene chips of MiRCURY^TM probe were used to detect and compare the microRNA expression of the lip and palate tissues in 4 NSCL/P patients as well as umbilical cord tissues in 4 normal newborns. Results: In total, 254 differentially expressed miRNAs were detected, among which 181 up-regulate and 73 down-regulate (P<0.05). According to the fold change and P value, the top ten different miRNAs were selected for target prediction. The results showed that hsa-miR-1260b, hsa-miR-205-5p, hsa-miR-24-3p, hsa-miR-27b-3p and hsa-miR-720 are combined with Wnt5a, Wnt9b and Wnt10a. Conclusion: Differentially expressed miRNA may control the development of lip and palate by targeting Wnt family in embryonic period.