不育男性精子中的顶体酶活性及精液参数研究

目的研究不育男性精子中顶体酶活性与精液参数。 方法选取我院收治的不育男性140例作为研究对象，再按照顶体酶参数是否正常划分为两组，观察组1（n=68）为精液参数正常组，即精液量、液化时间、外观、精子活力、外形以及数量等均无明显异常，观察组2（n=72）为精液参数异常组，即抗精抗体为阳性或上述检查有不少于1项明显异常，且存在生殖系统急慢性炎症史；选取1年内正常生育男性70例作为研究对象，设为对照组。应用手淫法对所有男性精液予以收集，且应用改良Kennedy法检测精子顶体酶活性，对比3组男性精子顶体酶活性。结果观察组2（a+b）级精子活动率明显低于其他两组（P<0.05）；对照组精子浓度明显高于观察组（P<0.05）；对照组顶体酶活性明显高于观察组1与观察组2（P<0.05）。 结论顶体酶活性低下密切关联于男性不育，检测不育男性精子中顶体酶活性临床价值重大，可将精子质量全面反映出来，进而评价其功能状态，有推广价值。  

The acrosome enzyme activity and the semen parameters in infertile males

Objective To study sperm acrosome enzyme activity and sperm parameters in infertile men. Methods A total of 140 cases of infertile subjects were enrolled and divided into two observation groups by the parameter of acrosome enzyme. Semen parameters included shape and number of semen volume, liquefaction time, appearance, sperm motility. 68 subjects with completely normal semen parameters were selected as observation group 1, while the other 72 subjects in observation group 2 has a history of reproductive system inflammation and at least one abnormal semen parameters, or showed an obvious exception of anti-sperm antibodies (AsAb). Control group was set with 70 cases of normal fertile men. Sperm acrosome activity was analysed by improved Kennedy and compared between these three groups. Sperm samples were all collected by masturbation. Results Observation group 2 shows a significantly lower value of male sperm acrosome activity than the other two groups (P<0.05); Sperm concentration of the control group was (136.84±21.77) × 106/mL, higher than the observation group (P<0.05); Acrosome enzyme activity in control group was 92.64±18.75 iIU/106/mL, significantly higher than the observation group 1 (28.79 ± 3.05 iIU/106/mL) and observation group2 (16.47 ± 2.47 iIU/106/mL) (P<0.05). Conclusion Reduction of acrosome activity is closely associated with male infertility, Detection of sperm acrosome activity in infertile men , which can fully reflected in the quality of sperm, evaluation of their functional status, is of major clinical value.