羊纤维环部分缺损动物模型的构建

摘要： 目的 建立可用于椎间盘缺损修复研究的纤维环部分缺损动物模型。方法 使用 image J 1.46r 软件测量 5 根羊脊柱 X 线片 T12/L1~L6/S1 椎间隙高度， 轴向剖开 T12/L1~L6/S1 椎间盘， 测量拟作缺损处纤维环厚度， 使用 11 号尖刀在 3 根离体羊脊柱 T12/L1~L5/6 椎间盘左前方制作上底 3 mm、 下底 5 mm、 高 5 mm、 厚 3 mm， 下底朝向髓核的梯形缺损， 上述羊脊柱均取自 1 岁龄绵羊。采用微创外侧手术入路在 2 只 1 岁龄绵羊 T12/L1~L5/6 椎间盘左前方制作相同规格的梯形缺损。通过轴向剖开椎间盘测量梯形缺损的边长、 对切取的纤维环和少量髓核称质量， 以此评估所制作的椎间盘梯形缺损。结果 绵羊腰椎间隙高度为（4.45±0.28） mm， 拟作缺损处纤维环厚度为（4.08±0.50） mm， 分别与拟作梯形缺损的厚度 （3 mm） 和高度 （5 mm） 差异有统计学意义 （P < 0.05）； 离体羊腰椎间盘梯形缺损的上底（3.03±0.09） mm、 下底（5.03±0.09） mm、 高度（4.97±0.10） mm 和厚度（3.02±0.06） mm 分别与梯形缺损预定值（上底 3 mm、 下底 5 mm、 高度 5 mm 和厚度 3 mm） 之间的差异无统计学意义 （P > 0.05）； 从离体和在体的羊腰椎间盘梯形缺损处取出的纤维环及髓核质量分别为 （0.162±0.011） g 和 （0.166±0.014） g， 两者差异无统计学意义 （P＞0.05）。结论 通过微创外侧手术入路， 使用实验中制作梯形缺损的方法可以简单、 安全、 可靠地制作出符合要求、 能用于椎间盘缺损修复研究的羊纤维环部分缺损的动物模型。

Establishment of annulus fibrosus partial defect model in sheep

ponding Abstract： Objective To establish an animal model of annulus fibrosus (AF) partial defect for the repairing of interver? tebral disc (IVD) defect. Methods Image J 1.46r software was used to measure the T12/L1-L6/S1 intervertebral height in ovine lumbar spine X-ray films. AF thickness was measured by axial split disc. A 11 blade was used to make a trapezoid de? fect of upper bottom 3 mm, lower bottom 5 mm, height 5 mm and thickness 3 mm, whose lower bottom toward the nucleus pulposus (NP) in the left front of ovine lumbar IVD in vitro. The minimally invasive lateral approach was used to make the same type of trapezoid defect in the left front of the ovine lumbar IVD in vivo. The trapezoidal defect length of the axial divid? ing disc was measured, AF and a small amount of NP from trapezoidal defect in IVD were weighed, and the production of trapezoidal defect in IVD was evaluated. Results The lumbar intervertebral space height of ovine was (4.45±0.28) mm. There were significant differences in the thickness of AF (4.08±0.50) mm , thickness (3 mm) and height (5 mm) of trapezoidal defect (P < 0.05), respectively. There were no significant differences in trapezoidal defects in ovine lumbar IVD in vitro on the upper bottom (3.03±0.09)mm, the lower bottom (5.03±0.09) mm, the height (4.97±0.10) mm, the thickness(3.02±0.06) mm and the trapezoidal defect predetermined value on the upper bottom 3 mm, the lower bottom 5 mm, the height 5 mm and the thickness 3 mm (P > 0. 05). The weights of the AF and NP taken out from ovine lumbar IVD in vitro and in vivo were (0.162 ± 0.011) g and (0.166 ± 0.014) g, and there was no significant difference between them (P > 0.05). Conclusion Through the operation of minimally invasive lateral approach, the method of making a trapezoidal defect in the experiments can establish animal model of AF partial defect, which meets the requirements for the repairing of IVD defect, and is simple, safe and reliable