苯与甲醛联合染毒对小鼠睾丸及精子的损伤作用

目的 通过研究苯与甲醛联合染毒对雄性小鼠睾丸及精子的损伤情况，探讨苯与甲醛联合对其损伤的作用机制。 方法 选择40只SPF级成年雄性小鼠随机分为4组，分别为对照组(A组)、苯单独染毒组(B组)、甲醛单独染毒组(C组)、苯与甲醛联合染毒组(D组)，每组小鼠10只。A组小鼠灌胃生理盐水和植物油；B组灌胃200 mg/(kg.bw)剂量的苯；C组灌胃10 mg/(kg.bw)剂量的甲醛、D组灌胃苯200 mg/(kg.bw)+甲醛10 mg/(kg.bw)。各组均连续染毒5 d，再饲养30 d后称重处死动物，分离双侧睾丸测定其睾丸脏器系数、光学显微镜下观察小鼠精子数量、死亡精子和畸形精子；按照常规方法制作睾丸组织病理切片，HE染色后置显微镜下观察其睾丸组织病理变化；采用试剂盒检测睾丸T-AOC和Na+-K+-ATP酶活性；采用SPSS 17.0软件对实验数据进行统计分析。 结果 D组小鼠体重及睾丸脏器系数均分别低于A、B、C组(P<0.05)；与A及B、C组比较，D组小鼠精子数减少14.9/(107个·g附睾)]，精子的死亡率(45.17‰)、畸形率(58.33‰)均增加(P<0.05)；B、C、D三个染毒组小鼠睾丸组织均出现不同程度病理损害，其中D组病理损害明显；D组小鼠睾丸T-AOC、Na+-K+-ATP活性较B、C组降低，差异均有统计学意义(P<0.05)。 结论 甲醛与苯联合染毒对小鼠睾丸及精子有明显损伤作用；其损伤机制可能通过抑制ATP酶活性降低睾丸生殖细胞的抗氧化性有关。 

The reproductive toxicity of benzene and formaldehyde for male mice

Objective To explore the mechanism of benzene and formaldehyde joint damage through the study of benzene and formaldehyde jointly infected in male mice testis and sperm damage. Methods Fourty SPF male mice were randomly divided into 4 groups,control group(A group),benzene respectively separate infected group(B group),formaldehyde infected group(C group),benzene and formaldehyde jointly infected group(D group),10 mice in each group.The mice in A group were intragastric saline and vegetable oil.The mice in B group were intragastric benzene 200 mg/(kg.bw).The mice in C group were intragastric formaldehyde 10 mg/(kg.bw).The mice in D group were intragastric benzene with 200 mg/(kg.bw)+formaldehyde 10 mg/(kg.bw).Each groups were exposed for 5 days,then to weigh after 30 days and be killed,to separate bilateral testes measure its testicular viscera coefficient,optical microscope sperm counts,dead sperm and sperm deformity in mice.According to the conventional method of making testicular tissue pathological slices,HE dye rear under a microscope to observe the testicular tissue pathological changes.Use the kits testicular T-AOC and Na+-K+ atpase activity.The experimental data were analyzed using SPSS 17.0. Results Body weight and testis organ coefficient of D group exposed mice were lower than A,B,C group(P<0.05).Compared with the A group and B,C group,D group mice sperm count reduced14.9/(107·g epididymitis)],mortality(45.17%),deformity rate(58.33%) were increased(P<0.05);B,C and D group in mices,were showed different degree of pathological damages,the D group mice were damaged obviously;D groups mice testis T-AOC,Na+-K+-ATP were lower than the B,C groups,its difference has statistics significance(P<0.05). Conclusion The combined toxicity of benzene and formaldehyde have obvious synergistic effect on testis and sperm damage in mice;the injury may reduce the inhibiting ability of mice testicle cell and inhibiting ATP enzyme activity.