健康儿童及成人唾液微生物多样性研究

目的:16S rRNA高通量测序技术分析口腔健康儿童与成人唾液微生物多样性的差异。方法:选择11位受检者(5例口腔健康成人(AJ组),6例口腔健康儿童(CJ组))的唾液样本进行细菌DNA提取,后利用Illumina MiSeq测序平台对11例样本DNA的16S rRNA V4-V5区进行测序,并分析两组唾液样本间菌群多样性。结果:11个样本总获得 474119 条序列,共9223OTUs,归属于26个门,374个属;PcoA检测显示两组微生物群落结构有明显差异;T-检验显示两组间差异菌属为:纤毛菌属(Leptotrichia)、卟啉单胞菌属(Porphyromona)、密螺旋体属(Treponema)、孪生球菌属(Gemella)、颗粒链菌属(Granulicatella);其中,卟啉单胞菌属、密螺旋体属、纤毛菌属在AJ组的相对丰度高于CJ组,孪生球菌属、颗粒链菌属在CJ组相对丰度高于AJ组。结论:健康成人与健康儿童口腔唾液微生物群落结构在种类上相似,而在丰度上存在差异,差异提示:健康成人较健康儿童更倾向患牙周病;健康儿童较健康成人更倾向于患龋病。

Diversity of Salivary Microbiology in Healthy Children and Adults.

Objective: To analyse the differences of oral microbial diversity between healthy children and adults through high-throughput sequencing of 16S rRNA. Methods: Eleven specimens of saliva (5 from healthy adults, i.e. AJ group and 6 from healthy children, i.e. CJ group) were collected respectively. The DNA of microbes from saliva specimens was extracted, and the V4-V5 eras of 16S rRNA was sequenced by Illumina MiSeq sequencing platform. The diversity of microbial community structure from these two groups was analyzed. Results: The different genera between two groups were Leptotrichia, Porphyromona, Treponema, Gemella, and Granulicatella. Principal component analysis (PcoA) test showed that there were significant differences in microbial community structure between CJ group and AJ group. From 11 samples, 474,119 sequences and 9,223 operational takonomic units(OUTs)were obtained, which belonged to 26 phyla and 374 genera. Conclusion: The oral saliva microbial community structure in healthy adults and children is similar in kind but different in quantity. The differences suggest that healthy adults are more likely to get periodontal disease than healthy children while who are more likely to suffer from dental caries.