转基因番茄可食用防龋疫苗免疫BALB/c小鼠的实验研究

目的: 观察含PAcA/CTB的转基因番茄可食用防龋疫苗的免疫原性及免疫反应性。方法: 将18只6~8周龄的雄性BALB/c小鼠,随机分为3组,每周分别灌胃含有PAcA/CTB嵌合蛋白的转基因番茄果汁(实验组)、非转基因番茄果汁(阴性对照组)、灭活全菌疫苗(阳性对照组)。于首次免疫前和免疫后第1、2、3、4周采集血液、唾液样品。ELISA检测其血清和唾液样本中的抗变异链球菌PAcA的IgG、IgA抗体效价。结果: 阳性对照组和实验组小鼠血液和唾液中特异性抗体从免疫后1周开始升高,初始免疫后第4周达高峰,分别与阴性对照组比较存在统计学差异(P<0.05或P<0.01)。免疫后第2、3、4周阳性对照组与实验组差异有统计学意义(P<0.05)。结论: 转基因番茄所表达的外源目的蛋白具有抗原性,能够诱导BALB/c小鼠产生免疫应答。

An Experimental Study of Edible Transgenic Tomatoes Vaccine against Caries in BALB/c Mouse.

Objective: To observe the immunogenic and immunoreactivity of transgenic tomatoes vaccine containing PAcA/CTB against caries by animal experiment. Methods: Eighteen androgenesis BALB/c mice of six to eigh-week-old were randomly divided in three groups and fed transgenic tomatoes fruit tissue (experiment group), control tomatoes fruit tissue (negative control group) or deactivated S.mutans (positive control group). Animals were bled and saliva samples were collected one day before the first immunity and at the 1, 2, 3, 4th week after immunity. Antibody titer was analyzed by ELISA to observe the immunogenic antibody. Results: The PAcA specific IgG antibodies in sera hoisted in the positive control group and the experiment group one week after the first immunity.The antibody titer was significantly higher than those fed with control transgenic tomatoes (P<0.05 or P<0.01) at the 4th week. The antibody titer was significantly different between positive control group and the experiment group at the 2nd, 3rd and 4th weeks after the first immunity. Conclusion: The foreign protein which induced by transgenic tomatoes was immunogenic and could induce immunity responses in BALB/c mice.