郁金提取物对CCI大鼠脊髓P-P38表达的影响

目的 探讨不同剂量郁金提取物对神经病理性疼痛模型慢性压迫性神经损伤(chronic constriction injury,CCI)大鼠痛阈及脊髓早晚期因子P-P38表达的影响及调节作用，并讨论郁金提取物对神经病理性痛的影响机制。 方法 选择成年健康雄性SD大鼠36只，体重250～294 g，建立CCI大鼠模型，将SD大鼠随机分为:正常组，模型组，郁金提取物低、中、高剂量组，加巴喷汀组和溶媒组，每组4只，对各组大鼠进行热缩足潜伏期测定，用蛋白免疫印迹(Western blot)法检测大鼠L₄～L₆脊髓的中早晚期因子P-P38表达。 结果 ①与正常组相比，造模后大鼠热缩足潜伏期(TWL)值显著下降(P<0.05)，说明神经病理性模型造模成功；与溶媒组相比，郁金提取物高剂量组能显著上调CCI模型大鼠(Thermal withdrawal latency,TWL)值(P<0.01)。②造模后大鼠L₄～L₆脊髓的中早晚期因子P-P38表达灰度值与内参的比值成上升趋势，均较正常组显著增加(P<0.01)；与溶媒组相比，郁金提取物高剂量组和加巴喷汀组能显著降低L₄～L₆脊髓的中早晚期因子P-P38的表达(P<0.01)。 结论 高剂量郁金提取物可能通过降低神经病理性疼痛CCI模型大鼠脊髓的中早晚期因子P-P38的表达而发挥抑制神经病理性疼痛的产生和发展。 

The effect of radix curcumae extract on expression of P-P38 in spinal cordsof CCI rats

Objective To investigate the effect of different doses of Radix curcumae extract onexpression of P-P38and pain threshold of CCI rats. Methods Thirty-six healthy adult male SD rats,weight 250-250 g,were chosen.To establish the CCI rat model,the SD rats were divided into normal group,model group,radix curcumae extract low,middle and high dose group,and gabapentin group and solvent group,and all of rats in each group were measured thermal paw withdrawal latency,using Western blot detection of rat L₄-L₆ spinal cord of P-P38 expression. Results ①Compared with the normal group,rats TWL value decreased significantly(P<0.05),indicating that neuropathic model was successfully built.Compared with the solvent group,Curcuma aromatica extract high dose group can significantly increased in CCI rats TWL value(P<0.01).②After modeling,L₄-L₆ spinal cord in rats of P-P38 expression of gray value and the ratio of reference into an upward trend,compared with normal group increased significantly(P<0.01);compared with the solvent group,curcuma aromatica extract high dose group and gabapentin group can significantly reduce the L₄-L₆ spinal cord of P-P38 expression(P<0.01). Conclusion High dose of Radix curcumae extract may play a role in neuropathic pain through reducing the expression of P-P38 in spinal cord of CCI model rats.