PinX1在胃癌中的表达及其对胃癌细胞生物学行为的影响

目的 探索PinX1基因在胃癌中的表达情况，并观察PinX1表达水平改变对胃癌细胞恶性生物学行为的影响。 方法 通过PinX1质粒转染及RNAi干扰技术，分别上调和下调胃癌细胞中的PinX1基因表达水平，然后运用Western Blot技术在蛋白水平检测正常胃癌细胞、PinX1基因上调组和PinX1基因敲低组胃癌细胞目标蛋白PinX1的表达情况，并通过CCK8法检测不同处理组胃癌细胞增殖曲线。选取36对胃癌组织和癌旁正常胃黏膜的组织，检测PinX1的表达情况并计算其与肿瘤分期之间的相关性。采用SPSS软件进行统计分析并绘制统计图。 结果 胃癌细胞系SGC7901及MKN28中PinX1的表达水平低于正常胃黏膜细胞株GES-1中PinX1的表达水平，PinX1基因在MKN28转染组胃癌细胞较正常MKN28细胞中表达上调，MKN28胃癌细胞生长速度相应的减缓(P＜0.05)，而干扰组胃癌细胞PinX1基因表达下调，SGC7901胃癌细胞生长速度相应的增快(P＜0.05)。PinX1在胃癌组织中的表达率低于癌旁组织的表达率(P＜0.05)，且PinX1表达水平与胃癌T分期呈现出一定的相关性。 结论 PinX1在胃癌组织和胃癌细胞中低表达并负性调控胃癌细胞恶性生物学行为。 

Expression of PinX1 gene in gastric cancer and its impact on the biological behavior of gastric tumor cells

Objective To explore the expression of Pin X1 in gastric cancer and its impact on the biological behavior of gastric tumor cells. Methods Plasmid transfection was performed to up-regulate the expression of Pin X1,and RNA interference was performed to down-regulate the expression of Pin X1 in gastric cancers. Then western blotting was used to detect the expression of Pin X1 in normal gastric cancer cells,cells with Pin X1- Plasmid transfection and cells with RNAi.CCK8 assay was used to describe cell growth curve of different group cells. Pin X1 expression was also detected by WB in36 pairs of gastric cancer tissues and normal gastric mucosa tissues. The correlation between tumor staging and Pin X1 expression was also calculated. The data was analyzed by SPSS(version 17. 0),and Chi-square test and T test were used to compare the differences between different groups of qualitative or quantitative data. P < 0. 05 was statistically significant. Results The expression of Pin X1 in SGC7901 and MKN28 was lower than in the normal gastric cell line GES-1. After plasmid transfection,Pin X1 was up-regulated in MKN28 cells,which made the growth slower than normal MKN28 cells(P < 0. 05). After RNA interference,Pin X1 was down-regulated in SGC7901 cells,which made the growth faster than normal SGC7901cells(P < 0. 05). The positive rate of Pin X1 expression in gastric cancer tissues was lower than in the adjacent normal mucosa tissues(P < 0. 05),and was related to T staging. Conclusion The expression of Pin X1 in gastric cells and tissues is lower,which may play a negative role of regulation of the malignant behavior of gastric cancer cells.