miR-155在口腔扁平苔藓患者外周血及组织中表达及意义

目的:探讨miR-155在口腔扁平苔藓患者外周血及组织中表达及意义。方法:选取85例初诊口腔扁平苔藓患者,依据患者病损基部黏膜状况不同分为非糜烂型(n=48)和糜烂型(n=37),选取40例健康体检者作为对照组,以及正常黏膜组织45份。利用实时荧光定量PCR对患者和对照组血浆及外周血单个核细胞中miR-155表达进行检测,并对患者黏膜组织和正常黏膜组织中miR-155表达进行检测,利用ROC曲线分析miR-155表达在预测患者病损程度中的效能。结果:口腔扁平苔藓患者血浆和外周血单个核细胞中miR-155相对表达量均高于对照组,口腔扁平苔藓患者组织中miR-155相对表达量(1.47±0.34),显著高于正常黏膜组织的(0.95±0.12),差异均有统计学意义(P<0.05);miR-155在糜烂型口腔扁平苔藓患者血浆和组织中相对表达量均高于非糜烂型口腔扁平苔藓患者,差异均有统计学意义(P<0.05);ROC曲线分析显示,口腔扁平苔藓患者血浆中miR-155相对表达量在预测病损程度曲线下面积0.807(95%CI:0.712～0.903),灵敏度67.6%,特异度85.4%,口腔扁平苔藓患者组织中miR-155相对表达量在预测病损程度曲线下面积0.845(95%CI:0.761～0.928),灵敏度70.3%,特异度87.5%。结论:miR-155可作为患者病损严重程度的预测指标。

Expression of miR-155 in Peripheral Blood and Tissues of Oral Lichen Planus Patients.

Objective: To investigate the expression of miR-155 in peripheral blood and tissues of oral lichen planus patients. Methods: Eighty-five oral lichen planus patients were selected. They were divided into non-erosive type (n=48) and erosive type (n=37), according to the different conditions of the base of mucosa of lesions. Forty healthy subjects and forty-five normal tissues were selected as control group. The expressions of miR-155 in plasma and mononuclear cells in patients and the control group were detected by real-time quantitative PCR. And the expressions of miR-155 in mucosa of patients and normal mucosa were also detected. The efficacy of miR-155 in predicting the extent of lesions in patients was analyzed using ROC curve. Results: The relative expression levels of miR-155 in plasma and mononuclear cells of oral lichen planus patients (1.47±0.34) were significantly higher than the control group (0.95±0.12) (P<0.05). The relative expressions of miR-155 in plasma and tissue of erosive type group were higher than the non-erosive type group (P<0.05). ROC curve analysis showed that the relative expression of miR-155 in plasma of oral lichen planus patients in predicting the extent of the lesion was 0.807 (95%CI: 0.712-0.903). The sensitivity was 67.6% and specificity was 85.4%. The relative expression of miR-155 in tissues of oral lichen planus patients in predicting the extent of the lesion was 0.845 (95%CI: 0.761-0.928). The sensitivity was 70.3% and specificity was 87.5%. Conclusion: miR-155 might be used as a predictor index for the severity of the patient's lesions.