下调 HER2 降低自噬活性抑制人肺癌细胞增殖 转移作用的体外研究

目的 探讨下调 HER2 表达是否抑制细胞自噬活性， 从而影响人肺癌细胞的增殖、 迁徙和侵袭。方法 采用浓度 5 μmol/L 自噬抑制剂 3-MA、 10 μmol/L HER2 抑制剂 Neratinib 分别作用人肺癌细胞 A549。Western blot 检测肺癌细胞 A549 中 HER2、 Beclin-1 及 LC3B （Ⅱ/Ⅰ） 蛋白表达水平； Wound healing 和 Transwell invasion 实验检测 细胞迁徙、 侵袭能力； 四甲基偶氮唑盐 （MTT） 检测细胞的增殖能力。结果 Neratinib 和自噬抑制剂 （3-MA） 作用 24 h 后， 细胞 HER2、 Beclin-1 及 LC3B（Ⅱ/Ⅰ）蛋白表达水平显著低于阴性对照组； Neratinib、 自噬抑制剂（3-MA）处理组 的细胞增殖、 迁徙和侵袭能力显著低于阴性对照细胞。结论 下调 HER2 能降低人肺癌细胞 A549 的自噬活性并抑 制其增殖、 迁徙和侵袭

The inhibition of autophagy suppresses proliferation and metastasis of lung cancer cells via down-regulation of HER2

Objective To investigate whether the down-regulation of HER2 can inhibit the autophagy of cells and influence the proliferation and metastasis of lung cancer cells. Methods The 5 μmol/L of autophagy inhibitor (3-MA) and 10 μmol/L of HER2 inhibitor (Neratinib) were used to treat human lung cancer A549 cells. Western blot assay was used to detect the protein expressions of HER2, Beclin-1 and LC3B (Ⅱ/Ⅰ) in A549 cells. Wound healing and Transwell invasion methods were used to detect the invasion and migration ability of A549 cells. MTT assay was performed to evaluate the cell proliferation. Results Western blot analysis showed that the protein expressions of HER2, Beclin-1 and LC3B (Ⅱ/Ⅰ) were significantly declined in A549 cells treated with Neratinib and 3-MA for 24 h compared to cells in control group. The cell proliferation, migration and invasion ability were significantly decreased in A549 cells treated with Neratinib and 3-MA thanthoseofcontrolcells.Conclusion TheinhibitionofHER2proteincansuppresstheautophagyandproliferationofA549cells.