N-乙酰基转移酶10蛋白和朊蛋白在口腔鳞状细胞癌组织中的表达及相关性研究

目的:检测N-乙酰基转移酶10蛋白(Naa10p)和朊蛋白(PrPc)在口腔鳞状细胞癌(OSCC) 、白斑、口腔正常黏膜中的表达及临床意义。方法:应用免疫组化EnvisionTM法检测OSCC(112例)、白斑(42例)及正常黏膜组织(11例)中Naa10p和PrPc的表达情况,并分析其与临床病理特征相关性。结果:Naa10p和PrPc在OSCC表达最高,白斑次之,正常黏膜组织中表达最低。Naa10p在3种不同组织中的表达两两比较均有显著统计学差异(P<0.05),PrPc分别在白斑和OSCC组织,正常口腔黏膜与OSCC组织中的表达有统计学差异(P<0.05)。Naa10p的表达水平与OSCC的TNM分期、淋巴结转移、组织学分化程度密切相关(P<0.05),与性别、年龄无关。PrPc表达仅与组织学分化程度密切相关(P<0.05)。PrPc在OSCC中的表达强度随组织学分化程度下降而明显升高(P<0.05),而Naa10p的表达强度随组织学分化程度下降而明显下降(P<0.05)。结论:Naa10p和PrPc与OSCC的发生和转移相关。

Expression and Correlation of Naa10p and PrPc in Oral Squamous Cell Carcionoma.

Objective: To detect the expression and clinical significance of N-α-acetyltransferase10 protein (Naa10p) and cellular prion protein (PrPc) in oral squamous cell carcinoma (OSCC), oral leukoplakia and normal oral mucosa. Methods: The expressions of Naa10p and PrPc were detected by immunohistochemistry assay (EnvisionTM) in OSCC (112 cases), leukoplakia (42 cases) and normal mucosa tissues (11 cases), and the correlation between their expression and clinical features was analyzed. Results: The expression level of Naa10p and PrPc was the highest in OSCC, followed by leukoplakia, and the lowest in normal mucosa tissues. Naa10p expression in three different tissues was statistically significant (P<0.05). PrPc expression was significantly different in OSCC compared to oral leukoplakia and normal tissues (P<0.05), while there was no difference between oral leukoplakia and normal mucosa. In addition, Naa10p expression was correlated with TNM stage, lymph node metastasis and cell differentiation of patients with OSCC (P<0.05), but was not associated with gender and age. Moreover, PrPc expression was only positively correlated with cell differentiation in OSCC (P<0.05), while Naa10p expression was negatively correlated with cell differentiation (P<0.05). Conclusion: Naa10p and PrPc are associated with the tumorgenesis and metastasis of OSCC.