布伦登卢普沙门菌耐药性与毒力基因分析

目的 研究临床分离的非伤寒沙门菌布伦登卢普血清型（布伦登卢普沙门菌）对抗菌药物的耐药性及其 携带的毒力基因特征。方法 收集 2012—2014 年每年 4—10 月天津市两家教学医院肠道门诊急性腹泻患者的临 床资料， 将急性腹泻患者粪便标本进行沙门菌分离培养、 生化及 PCR 鉴定、 血清学分型， 对得到的布伦登卢普沙门菌 进行抗菌药物敏感性检测、 PCR 扩增沙门菌毒力岛 （SPI） 1~5 的代表性基因和 SPI 的调节基因。结果 3 年共检测到 非重复非伤寒沙门菌 153 株， 其中 8 株 （5.23%） 为布伦登卢普沙门菌。8 株 invA-PCR 鉴定均呈阳性， 对萘啶酸耐药 率 100%， 对环丙沙星和左氧氟沙星中介耐药率 100%， 对其余检测的抗菌药物敏感； 8 株均检测到了 SPI 1~5 代表性 基因和 SPI 调节基因 （sitC、 hilA、 sseL、 sifA、 mgtC、 siiE、 sopB 和 phoP）。结论 天津地区布伦登卢普沙门菌临床株对氟 喹诺酮耐药并携带 SPI 1~5 毒力基因与调节基因， 对公众健康构成潜在威胁， 应持续开展相关监测与研究。

Antimicrobial resistance and virulence gene profiling of Salmonella enterica serovars Braenderup

Objective To investigate antimicrobial resistance and virulence gene profiles of clinically isolated nontyphoidal Salmonella enterica serovars Braenderup (S. Braenderup). Methods Clinical date of patients with acute diarrhea from two teaching hospitals in our city were collected from April to October of 2012 to 2014. Salmonella from stool samples was screened by culture, and identified by biochemical, PCR and serotype methods. Isolates of S. Braenderup were examined with antibiotic susceptibilities. PCR amplification was used for representative genes of Salmonella pathogenicity islands (SPI) 1-5 and SPI regulator. Results Among 153 non-repetitive NTS isolates in the three years, 8 isolates (5.23%) of S. Breanderup were identified. All 8 isolates were positive with invA gene. The resistance rate to nalidixic acid was 100%, and the same with the intermediate resistance rate to ciprofloxacin and levofloxacin. But they were susceptible to other antimicrobial agents. The 8 isolates were positive with the representative genes of SPI 1-5 and regulators (sitC, hilA, sseL, sifA, mgtC, siiE, sopB and phoP). Conclusion Clinical isolates of S. Braenderup in Tianjin are resistant to fluoroquinolones and harbored genes of SPI 1-5 and regulator, and which can threaten public health. Continuous surveillance and research should be carried out in our city.