| E-钙黏素mRNA在大鼠舌黏膜癌变过程中的表达研究 |
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| 引用本文： | 谭红,毛亮,王敏,廖鹏程,聂敏海.E-钙黏素mRNA在大鼠舌黏膜癌变过程中的表达研究[J].口腔医学研究,2016,32(1):31. |
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| 作者姓名： | 谭红 毛亮 王敏 廖鹏程 聂敏海 |
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| 作者单位： | 1. 四川医科大学口腔医学院 四川 泸州 646000;2. 四川医科大学口颌面重建和再生实验室 四川 泸州 646000;3. 四川医科大学医学电生理学教育部重点实验室 四川 泸州 646000 |
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| 基金项目： | 四川省应用基础研究计划项目(编号:2013JY0126) |
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| 摘 要： | 目的:研究上皮型黏附分子E-钙粘素mRNA在大鼠舌黏膜癌变过程中的表达及其意义。方法:采用4NQO饮水法诱导大鼠舌鳞状细胞癌发生,实时荧光定量PCR技术检测组织标本中E-钙粘素mRNA的表达情况。结果:在大鼠舌鳞状细胞癌发生过程中E-钙粘素mRNA表达降低;上皮单纯增生组、轻度上皮异常增生组、中度和重度上皮异常增生组、鳞状细胞癌组4组标本中E-钙粘素 mRNA的表达量分别是上皮正常组标本中的0.453541倍、0.207062倍、0.190954倍、0.180987倍,且鳞状细胞癌组与上皮正常组间的差异具有统计学意义。结论:在大鼠舌黏膜癌变过程中E-钙粘素mRNA表达随着病理分级的增加呈逐渐降低的趋势。E-钙粘素mRNA表达变化是大鼠舌黏膜癌变过程中的早期事件。
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| 关 键 词： | 舌鳞状细胞癌 E-cad mRNA q-RT-PC |
| 收稿时间： | 2015-08-13 |
Expression of E-cadherin mRNA in the Rat Tongue Carcinogenesis. |
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| TAN Hong,MAO Liang,WANG Min,LIAO Peng-cheng,NIE Min-hai..Expression of E-cadherin mRNA in the Rat Tongue Carcinogenesis.[J].Journal of Oral Science Research,2016,32(1):31. |
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| Authors: | TAN Hong MAO Liang WANG Min LIAO Peng-cheng NIE Min-hai |
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| Institution: | 1. Stomatology of Sichuan Medical University, Luzhou 646000, China; 2. Laboratory of Oral and Maxillofacial Reconstruction and Regeneration, Sichuan Medical University, Luzhou 646000, China; 3. Key Laboratory of Medical Electrophysiology, Ministry of Education, Sichuan Medical University, Luzhou 646000, China |
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| Abstract: | Objective: To investigate the expression and significance of E-cadherin mRNA in the rat tongue carcinogenesis. Methods: 4NQO Drinking Water Act was used to induce tongue squamous cell carcinoma of rat. The mRNA expression level of E-cadherin was analyzed by q-RT-PCR. Results: The mRNA expression of E-cadherin was down-regulated in the rat tongue carcinogenesis. In the epithelial hyperplasia tissues, mild epithelial dysplasia tissues, moderate to severe epithelial dysplasia tissues and squamous cell carcinoma tissues, E-cadherin mRNA level was 0.453541, 0.207062, 0.190954, 0.180987 times by the normal epithelial tissues, respectively. The differences between squamous cell carcinoma group and normal epithelial group were statistically significant (P＜0.05). Conclusion: With the increase of pathological grade, E-cadherin mRNA expression showed a decreasing trend. The change of E-cadherin mRNA expression was an early event in rat tongue mucosa carcinogenesis. |
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