ICAM-1 K469E基因多态性对汉族人脑梗死形成的作用研究

目的 研究分析ICAM-1K 469E基因多态性与汉族人脑梗死形成的关系。 方法 选取2009年7月—2013年10月汉族住院脑梗死患者213例，采用聚合酶链反应和限制性片段长度多态性分析(PCR-RFLP)检测确定基因型。对照组224例，均为同期汉族健康体检者，年龄、性别与脑梗死组相匹配，各试验指标检测同脑梗死组。 结果 脑梗死不同面积与ICAM-1指标关系,脑梗死组(大面积)的ICAM-1指标明显高于脑梗死组(小面积)和脑梗死组(腔隙性)，比较差异具有统计学意义(P＜0.05)；脑梗死组(小面积)和脑梗死组(腔隙性)的ICAM-1指标差异无统计学意义(P＞0.05)；2组基因型、等位基因分布比较,ICAM-1基因型中，脑梗死组KK基因型显著多于对照组，EE基因型略低于对照组，比较差异具有统计学意义(P＜0.05)；2组EK基因型比较差异无统计学意义(P＞0.05)；2组等位基因型分布比较，K/E脑梗死组显著高于对照组，比较差异具有统计学意义(P＜0.05)。 结论 汉族人脑梗死患者与ICAM-1K469E基因多态性存在明确相关性，并且脑梗死面积的不同也与ICAM-1指标相关联，E等位基因可能是影响汉族人脑梗死的重要遗传基因。 

Association of ICAM-1 K469E polymorphism with cerebral infarction in Han nationality in China

Objective To study the association of ICAM-1 K469E polymorphism with cerebral infarction in Han nationality in China. Methods All 213 cases of cerebral infarction(CI group)and 224 people in physical examination(control group)in our hospital from July 2009 to October 2013 were enrolled into this study.All the subjects were compatible in nationality,age abd gender.The polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP)was performed to determine the ICAM-1 K469E polymorphism. Results The areas of cerebral infarction was related to ICAM-1 polymorphism:the index in the extensive cerebral infarction group was significantly higher than that in the small cerebellar infarcts group and lacunar cerebral infarction group,the difference was statistical significance(P<0.05);there was no statistical significance between the small cerebellar infarcts group and lacunar cerebral infarction group(P>0.05).For ICAM-1 genotypes,KK in CI group was was significantly more than that in the control group,the differences was statistically significant P<0.05);EE was slightly lower as compared with the control group;there was no statistically significant in the EK genotype between the two groups(P>0.05);for the alleles,K/E in the CI group was much higher when compared with the control group,the difference was statistically significant(P<0.05). Conclusion The morbidity and areas of cerebral infarction in Han nationality was obviously correlated to ICAM-1K 469E polymorphism,E allele may be the importsant gene for the cerebral infarction in Han nationality in China.