淋球菌外膜蛋白Porin I多抗的黏附抑制作用研究

目的:研究兔抗淋球菌外膜蛋白Porin I(P I)的多克隆抗体阻断淋病奈瑟菌对泌尿生殖道上皮的黏附作用。方法:将自行构建表达的淋球菌GST-P I融合蛋白作为抗原免疫新西兰兔,获得兔抗P I蛋白的多抗血清,纯化后得到P I-IgG抗体。建立淋球菌感染BALB/c小鼠模型,并通过观察阴道黏膜改变、分泌物涂片、冲洗液培养及阴道组织病理变化评价兔抗P I-IgG抗体对淋球菌黏附的影响。结果:经1 m g/m l兔抗P I-IgG处理后3 h再接种淋球菌的BALB/c小鼠生殖道黏膜未见红肿及脓液,分泌物涂片及阴道冲洗液未检及淋球菌,阴道组织病理检查也未见炎症细胞浸润。而低于浓度1 m g/m l及长于3 h兔抗P I-IgG处理的小鼠阴道组织病理可检及炎症细胞,但其它检查结果阴性。结论:纯化的抗淋球菌GST-P I融合蛋白的多克隆抗体,能有效抑制淋病奈瑟菌对小鼠泌尿生殖道上皮的黏附与感染,阻断作用及持续时间与抗体浓度有关。

Adhesional inhibiton of polyclonal anti-porin I antibody

Objective: To investigate the blockness effects of purified polyclonal anti-porin I antibody on N.gonorrhoeae adherence to genitourinary tract epithelia of BALB/c mouse.Methods: Polyclonal anti GST-PI antibody was generated by immunizing rabbit with GST-PI fusion protein which was constructed and expressed by ourselves.The purified immunoglobulin G was obtained by ammonium sulphate deposition and DEAE cellulose chromatography.Mice model of gonorrhea was established.In order to evaluate the effects of PI-IgG on gonococcus adhesion to vagina mucus,the macroscopic and pathological assessing as well as gonococcus culture was employed after gonococcus challenge on PI-IgG immunized mice.Results: No pus and pathological inflammation were observed on mice vagina mucus treated with 1 mg/ml PI-IgG 3 hours before gonococcus challenge.Gonococcus could not be detected in the smears and washing solutions from vagina.Pathological inflammation was found in mice treated with anti PI-IgG,in which the concentrations were lower than 1 mg/ml or the treated time was longer than 3 hours prior to gonococcus challenge.Conclusion: The purified anti PI-IgG can effectively inhibit the adherence and infection of gonococci to genitourinary tract epithelia of BALB/c mice.In addition,the blocking duration of anti PI-IgG is associated with antibody concentration.