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| 酶联免疫斑点法检测结核性胸水T淋巴细胞分泌IFN-γ的免疫功能 | |
| 引用本文： | 黄汉林,刘畅,董璇,杨炯.酶联免疫斑点法检测结核性胸水T淋巴细胞分泌IFN-γ的免疫功能[J].武汉大学学报(医学版),2009,30(3). |
| 作者姓名： | 黄汉林 刘畅 董璇 杨炯 |
| 作者单位： | 1. 武汉大学中南医院呼吸内科,湖北,武汉,430083;湖北省襄樊市第一人民医院ICU科,湖北,襄樊,441000 2. 武汉大学中南医院ICU ,湖北,武汉,430083 3. 武汉市结核病医院,湖北,武汉,430083 4. 武汉大学中南医院呼吸内科,湖北,武汉,430083 |
| 摘 要： | 目的:研究结核性胸膜炎患者胸水T淋巴细胞(TLC)特异性分泌γ-干扰素(IFN-γ)的免疫反应,为结核性胸水的诊断提供依据。方法:应用淋巴细胞分离技术分离13例结核性胸水和11例恶性胸水TLC,体外利用PPD抗原刺激,用酶联免疫斑点法(ELISPOT)检测两组胸水TLC分泌IFN-γ的免疫反应。每例患者院内常规做PPD试验。结果:结核性胸水TLC分泌IFN-γ的免疫反应阳性率为84.62%(11/13例),明显高于恶性胸水组18.18%(2/11例),两者之间差异有显著性(P<0.01)。ELISPOT和PPD试验的敏感性分别为84.62%,46.15%,两者之间有明显差异性(P<0.01)。而ELISPOT和PPD试验的特异性分别为81.82%,90.91%,两者之间差异不明显。结论:ELISPOT检测结核性胸膜炎的敏感性和特异性均较高,有助于临床上对结核性胸水的鉴别诊断。 |
| 关 键 词： | 结核性胸膜炎 γ-干扰素 T淋巴细胞 PPD抗原 酶联免疫斑点法 |
T-Lymphocytes Immune Function of IFN-Gamma Production from Tuberculous Pleural Effusions Assayed by ELISPOT |
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| HUANG Hanlin,LIU Chang,DONG Xuan,YANG Jiong.T-Lymphocytes Immune Function of IFN-Gamma Production from Tuberculous Pleural Effusions Assayed by ELISPOT[J].Medical Journal of Wuhan University,2009,30(3). | |
| Authors: | HUANG Hanlin LIU Chang DONG Xuan YANG Jiong |
| Institution: | HUANG Hanlin1,2,LIU Chang3,DONG Xuan4,YANG Jiong11Dept.of Respiratory,3Dept.of Intensive Care Unit,Zhongnan Hospital of Wuhan University,Wuhan 430071,China 2Dept.of Intensive Care Unit,the First People's Hospital of Xiangfan,Xiangfan 441000,China4Tuberculous Hospital of Wuhan,Wuhan 430083,China |
| Abstract: | Objective: To investigate the tuberculous specific interferon-gamma(IFN-γ) production of T-lymphocytes(TLCs) from Pleural effusion of tuberculosis and its significance for diagnosis.Methods: TLCs in tuberculous pleural(n=13) and malignant(n=11) effusions were obtained by lymphocyte isolate technique,and stimulated with PPD antigen in vitro.The production of IFN-γ was measured by enzyme link immunospot(ELISPOT).Each patient adopted the PPD test in hospital routinely.Results: The positive responsive rate of IFN-γ production by TLCs from tuberculous pleural effusions was 84.62%(11/13),and was significantly higher than 18.18%(2/11)(P<0.01) from malignant ones.The sensitivity of ELISPOT was 84.62% and higher than 46.15% of PPD test(P<0.01).But the specificity of ELISPOT was 81.82% and was not significantly different from that of PPD test 90.91%(P>0.05).Conclusion: ELISPOT can help differential diagnosis of tuberculous pleural effusions for its higher sensitivity and specificity. |
| Keywords: | Tuberculous Pleurisy Interferon-Gamma T-Lymphocytes PPD Antigen Enzyme Link Immunospot |
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