诱导兔骨髓间充质干细胞成为内皮细胞

目的] 研究骨髓间充质干细胞（mensenchymal stemcells，MSCs）向内皮细胞分化的可行性。方法] 密度梯度离心法分离兔骨髓间充质干细胞行体外培养和连续传代，获得较纯的MSCs。采用第2代的MSCs，以血管内皮细胞生长因子（VEGF）、碱性成纤维细胞生长因子（bFGF）进行体外诱导，倒置相差显微镜下观察细胞形态变化，免疫荧光方法鉴定内皮细胞，并通过透射电镜观察内皮细胞特异性WP小体。结果] 分化后的细胞在光镜下具有内皮细胞的形态学特征；免疫荧光染色证实实验组诱导后的细胞为内皮细胞。透射电镜显示诱导后细胞内出现内皮细胞特异性WP小体。【结论] 成体中的MSCs在一定诱导条件下可以表现内皮细胞样特征，是组织工程血管化理想的种子细胞。

Study on the possibility of differentiation of bone marrow mesenchymal cells into endithelial cells in vitro

Objective]To study the possibility of differentiation of rabbit marrow mesenchymal stem cells(MSCs)into endothelial cells in vitro.Method]BMSCs of rabbits were obtained by using gradient centrifuge method.The adhesive cells were preserved to passage culture to get pure MSCs.MSCs in the second generation were used.After induction with vascular endothelial growth factor(VEGF),microscopic exarm,cytoimmunofluorescent staining confirmd the trial group cells becomes to endothelial cells.W-P corpuscles which was the symbol of endothelial cells were observed by transmission electron microscope.Result]The differentiated cells demonstrated the characters of endothelial cells under phase contrast microscopy.The cytoimmunofluore-scent staining showed that the trial group cells were induced to endothelial cells.W-P corpuscles could be seen under transmission electron microscope.Conclusion]The adult rabbit BMSCs can present features of endothelial cells in certain induction medium,they are ideal donor cells of tissue engineering vascularization.