| 经冻存的APA微囊牛肾上腺髓质细胞功能研究 |
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| 引用本文： | 李涛,回建峰,杜智,宋继昌.经冻存的APA微囊牛肾上腺髓质细胞功能研究[J].国际生物医学工程杂志,2007,30(6):324-326. |
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| 作者姓名： | 李涛 回建峰 杜智 宋继昌 |
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| 作者单位： | 天津第三中心医院,300170 |
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| 摘 要： | 目的观察海藻酸钠-聚赖氨酸-海藻酸钠(alginate-polylysine-alginate,APA)微囊牛肾上腺髓质细胞经液氮冻存前后的微囊形态、细胞活性和儿茶酚胺分泌功能变化。方法APA微囊牛肾上腺髓质细胞(牛嗜铬细胞,bevine chromaffin cell,BCC)的冻存以DMSO为保护剂,冻存采取循序缓慢降温,复苏采取快速复温。通过微囊形态、细胞活性和儿茶酚胺分泌量检测观察细胞功能变化。结果与冻存前相比,冻存复苏后的APA微囊BCC形态及细胞活性无明显变化,儿茶酚胺分泌量约为冻存前的80%。结论经冻存复苏的APA微囊BCC仍然保持了较好的形态、细胞活性和儿茶酚胺分泌功能。
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| 关 键 词： | 海藻酸钠-聚赖氨酸-海藻酸钠微囊 牛肾上腺髓质细胞 冻存 儿茶酚胺 |
| 文章编号： | 1673-4181（2007）06-0324-03 |
| 收稿时间： | 2007-10-06 |
Function study on cryopreservation of APA microencapstulated bovine chromaffin cells |
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| LI Tao , HUI Jian-feng, DU Zhi,et al..Function study on cryopreservation of APA microencapstulated bovine chromaffin cells[J].International Journal of Biomedical Engineering,2007,30(6):324-326. |
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| Authors: | LI Tao HUI Jian-feng DU Zhi |
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| Institution: | Tianjin Third Central Hospital, Tianjin 300170, China |
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| Abstract: | Objective To observe the morphology, cell viability and secretion function of catecholamine of the alginate-polylysine-alginate (APA) microencapsulated bovine chromaffin cells (BCCs) before and after cryopreserving with liquid nitrogen. Methods The APA microencapsulated BCCs were cryopreserved with dimethyl sulfoxide as cryopreservative agent by slow cooling and rapid rewarming for revivification. The change of cell function was observed by detecting the cell viability and secretion of catecholamine. Resul... |
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| Keywords: | alginate-polylysine-alginate bovine chromaffin cell cryopreservation catecholamine release |
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