绞股蓝总皂甙和人参总皂甙对大鼠急性心肌缺血心脏舒张功能保护作用的比较

 目的：评价绞股蓝总皂甙(Gyp)对大鼠急性心肌缺血心脏舒张功能保护作用，并同人参总皂甙(Gin)做比较。方法：用SMUP-160心功能测定软件，分别测定正常对照，提前给予Gyp(iv,40 mg/kg)和Gin(iv，40 mg/kg)组大 鼠心脏的舒张功能。结果:Gyp(iv,40mg/kg)对麻醉大鼠基础心功能无影响。Gin(iV,40 mg/kg)可显著降低心室收 缩崃压（LVSP）和心率（HR）（P<0.05或P<0.01),一过性降低心室压最大上升和下降速率（士dP/dt_max)和心肌纤维零负荷下最大缩短速率(V_max)，对等容舒张期压力下降时间常数T无影响。结扎大鼠冠脉左前降支即刻引起左室舒张功能损伤并于10min达到峰值，表现为等容收缩相T值显著延长，-dP/dt_max和-dP/dt_max·LVSP^-1明显降低，心室舒张末压（LVEDP)则明显升高。冠脉结扎前给予Gyp或Gin可显著（P<0.05或P<0.01)拮抗T值的延长，LVEDP的抬高和-dP/dt_max,-dP/dt_max·LVSP^-1的降低。结论:Gyp和Gin对大鼠实验性心肌缺血心脏舒张功能均有明显的保护作用。同Gyp相比,Gin似能更有效地改善急性心肌缺血所致LVEDP的抬高。

Comparison of protective effects of gypenosides and ginsenosides on left ventricular relaxation during acute myocardial ischemia in rats

OBJECTIVE:To compare the protective effect of gypenosides(Gyp)on left ventricular relaxation with that of ginsenosides(Gin)during acute myocardial ischemia in rats. METHOD:By using SMUP-160 software,left ventricular relaxation of rat was determined among NS,Gyp(iv,40 mg/kg) and Gin(iv,40 mg/kg) Pretreated groups. RESULTS:Gyp (iv,40 mg/kg) had no effect on all the cardiac function parameters while Gin (iv,40 mg/kg) caused great(P<0.05 or P<0.01) decrease of LVSP and HR,transient decrease of ±dP/dt_max and V_max but no effect on T. Left ventricular relaxation was markedly impaired during acute myocardial ischemia in anesthetized rats with T prolonged, dP/dt_max and -dP/dt_max·LVSP^-1 declined as well as LVEDP elevated significantly. The impairment of left ventricular relaxation occurred after the onset of coronary artery occlusion and peaked at 10 min. Gyp and Gin administered prior to occlusion resulted in significant (P<0.05 or P<0.01) decrease of T,LVEDP and augment of -dP/dt_max·LVSP^-1 compared with NS group. CONCLUSION:Both Gyp and Gin had protective effects on left ventricular relaxation during acute myocardial ischemia in rats while Gin was more effective in ameliorating decrease of LVEDP induced by acute myocardial ischemia than Gyp.