赖型钩端螺旋体及澳洲型钩端螺旋体的ompL1和flaB2抗原基因序列分析

目的：构建棘型钩端螺旋体017及澳洲型钩端螺旋体607株外膜蛋白抗原基因ompL1和内鞭毛抗原基因flaB2的重组质粒，并分别对ompL1及flaB2基因进行序列分析。方法：通过聚合酶链反应扩增ompL1及flaB2，并将其分别克隆到pcDNA3.1/Myc-His(＋）载体T7启动子下游，构建抗原基因表达质粒，进行序列测定分析。结果：序列分析显示赖型钩体017株与澳洲型钩体607株的ompL1相同碱基949个（98．85％），碱基变异11个（1．15％）；flaB2的相同碱基823个（96．94％），碱基变异26个（3．06％），呈很高的保守性。结论：赖型钩体017株与澳洲型钩体607株的ompL1及flaB2分别具有高度同源性。

Sequence analysis of the gene encoding ompL1 and flaB2 from Leptospira interrogans serovar lai and Leptospira interrogans serovar australis

Objective To construct recombinant plasmids from Leptospira interrogans serovar lai strain 017 and Leptospira interrogans serovar australis strain 607 consisted of an outer membrane protein gene (ompL 1) and a flagellin B gene(flaB 2). The ompL 1 and flaB 2 gene were analyzed by DNA sequencing. Methods The DNA fragments of ompL 1 and flaB 2 were respectively amplified by PCR, then about cloned into the down stream of a T7 promoter of vector pcDNA3.1/Myc His(+), recombinant plasmids of the antigen gene were constructed, and analyzed by DNA sequencing. Results Analysis of the DNA sequence of ompL 1 from L.interrogans lai strain 017 and L.interrogans australis strain 607 showed that the similarity of nucleotide sequence was 98.85%(949/960), variation was 1.15%(11/960); About flaB 2, the similarity was 96.94%(823/849), variation was 3.06%(26/849), the sequences of each were highly conserved. Conclusion Sequences analysis of ompL 1 and flaB 2 from Leptospira interrogans serovar lai strain 017 respectively revealed a high level of identity with that from Leptospira interrogans serovar australis strain 607. [