溶组织内阿米巴ELISA试剂盒在阿米巴病调查的应用

目的了解景洪市爱尼族人群阿米巴病流行情况,为阿米巴病监测和防治提供依据,并评价ELISA试剂盒在阿米巴病调查应用的效果。方法以整群随机抽样爱尼族村寨,逐户收集新鲜粪便标本,用观察法、碘液涂片镜检法和TECHLAB第二代溶组织内阿米巴ELISA试剂盒对照检测粪便标本溶内阿米巴。结果镜检278例,E·h/E·d感染为9·71%;ELISA检测366例,E·h感染率为9·29%;镜检和ELISA共同阳性15例,占镜检阳性的55·56%;粪便观察和ELISA检测结果诊断5例为肠阿米巴病,发病率为1·37%,E·h致病率为14·71%。结论景洪爱尼族阿米巴病流行严重,应加强阿米巴病的监测和防治工作,同时积极推广新技术。

Epidemiological survey of amoebiasis by using TECHLAB E. HISTOLYTICAL Ⅱ ELISA kits

Objective To understand the prevalent situation of amoebiasis among Aini ethnic group in Jinghong City, Yunnan Province and provide basis for monitoring and control of the disease. Methods An epidemiological survey was carried out by random cluster sampling Aini ethnic group villages and fecal samples were collected house by house. The fecal samples were detected under microscope with iodine staining, TECHLAB 2~ nd generation Monoclonal ELISA kits. Results The infection rates of E.histolytica and E dispar were 9.71% among 278 persons examined by microscope with iodine staining. The infection rate of E.histolytica was 9.29% among 366 cases detected by ELISA. There were 15 ELISA positives among a total of 27 positives examined by microscope with iodine staining, E.histolytica accounted for 55.56% of E.histolytica/E dispar. 5 intestinal amoebiasis cases were diagnosed by stool examination and ELISA with an incidence of 1.37% and the pathogenic rate of E. histolytica was 14.71%. Conclusion Amoebiasis prevails seriously among Aini ethic group in Jinghong City. The control and monitoring measures should be strengthened and new diagnostic technique be applied simultaneously.