大鼠牙齿发育过程中骨涎蛋白和骨桥蛋白的表达

目的：探讨骨涎蛋白（bone sialoprotein BSP)和骨折蛋白（osteopontin,OPN)在大鼠牙齿发育中的时间和空间分布特点，及两者在矿化中的作用。方法：用免疫组化方法检测了SD大鼠出生后1d及1，2，3 ，5，8周的牙胚和颌骨中BSP及OPN的表达特点。结果：成熟的成釉细胞，成牙本质细胞，成牙骨质细胞和成骨细胞及基质中均表达BSP和OPN，牙骨质和牙槽骨中SBP和OPN的表达高于牙釉质的牙本质，在牙骨质和牙槽骨中，BSP在未矿化的基质和已矿化的基质中均有高表达，而OPN仅在矿化的前沿和新生线处有高表达。结论：BSP和OPN在大鼠牙齿及颌骨的形成和矿化中具有重要作用。尤其是OPN与牙釉质的形成也有重要关系。BSP和OPN的表达特点不同，提示二者在矿化组织的形成和矿化中的作用不同。

Expression of bone sialoprotein and osteopontin in developing dental tissues of rats

Objective To investigate the timing and location of the expression of bone sialoprotein(BSP) and osteopontin(OPN) in developing dental tissues of rats Methods Every three neonatal rats were sacrificed at day 1, weeks 1, 2, 3, 5 and 8 The mandibles were dissected and the first molar and the surrounding tissue were fixed, then demineralized with 15% EDTA Immunohistochemical technique was used to determine the expression of BSP and OPN in dental tissues and the surrounding bone at different time points Results Immunoreactivitis of BSP and OPN were present in matured ameloblasts, dentinoblasts, cementoblasts, osteoblasts, and the matrix The expression of BSP and OPN in cementum and alveolar bone was stronger than that in enamel and dentine In cementum and alveolar bone, BSP appeared to be concentrated in unmineralized and mineralized tissues, but OPN was concentrated in the mineralizing frontier and reversal line Conclusions BSP and OPN play an important role in the development and mineralization of rat mineralized tissues The expression of BSP was different from OPN, indicating their different functions