缺氧缺血性脑损伤大鼠Caspase-9的表达及高压氧干预疗效 |
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引用本文: | 王玲,刘俊艳,牛彦平,檀国军.缺氧缺血性脑损伤大鼠Caspase-9的表达及高压氧干预疗效[J].脑与神经疾病杂志,2007,15(4):247-249,257. |
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作者姓名: | 王玲 刘俊艳 牛彦平 檀国军 |
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作者单位: | 1. 河北医科大学第三医院神经内科 2. 河北医科大学第二医院 |
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摘 要: | 目的:探讨高压氧(HBO)对缺氧缺血新生大鼠模型神经细胞凋亡的影响,以及Caspase-9在该过程中的作用。方法:建立缺氧缺血(HIBD)大鼠模型,观察HBO对缺氧缺血性脑损伤的改善情况,并用免疫组化的方法检测Caspase-9表达在缺氧缺血脑组织中的动态变化。结果:HIBD模型组大鼠造模后18h、24h、48h、96h不同时间段患侧海马区及皮层区Caspase-9活性蛋白的表达均明显高于假手术组(P值均<0.05);HBO干预组大鼠海马区和皮层区各相应时间点Caspase-9活性蛋白表达均低于HIBD模型组大鼠(P值均<0.05),部分时间点接近假手术组基线水平。结论:HBO可以减轻新生大鼠HIBD模型神经细胞的凋亡;不同时程HBO干预治疗可降低HIBD模型大鼠皮层、海马神经细胞Caspase-9的表达活性。
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关 键 词: | Caspase-9 凋亡 高压氧 脑损伤 |
文章编号: | 1006-351X(2007)04-0247-03 |
收稿时间: | 2007-04-20 |
修稿时间: | 2007-04-20 |
The expression of Caspase-9 in the Hyperbaric Oxygen treatment on Hypoxic-Ischemic Brain Damage |
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Abstract: | Objectives: To explore the mechanism that how Hyperbaric Oxygen (HBO) reduces neurocyte apoptosis on rat subjects with Hypoxic-Ischemic Brain Damage (HIBD), and the effect of Caspase-9 expression in this process. Methods: To observe the effect of HBO in the Hypoxia-ischemia rat model, and check the dynastic change of Caspase-9 expression in the Ischemic Brain tissues by immunohistochemistry. Result: The expression of Caspase-9 in the hippocampus and cortex was higher than that of control at 18h, 24h, 48h and 96h time point. Compared with HIBD(P<0.05), the expression of Caspase-9 was significantly decreased at some time point, which is similar to that of baseline levels.(P<0.05)Conclusion: HBO can relieve the apoptosis of nerve cells on 7d-old HIBD rat model. HBO made the expression of Caspase-9 decreased on hippocampus and cortex at different time point. |
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Keywords: | Caspase-9 |
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