| AFP启动子调控增强型绿色荧光蛋白在人肝癌细胞中表达的研究 |
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| 引用本文： | 郝萍,王东林. AFP启动子调控增强型绿色荧光蛋白在人肝癌细胞中表达的研究[J]. 第三军医大学学报, 2004, 26(20): 1857-1859 |
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| 作者姓名： | 郝萍 王东林 |
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| 作者单位： | 第三军医大学新桥医院肿瘤科,全军肿瘤诊治中心,重庆市肿瘤生物技术研究所,重庆,400037;第三军医大学新桥医院肿瘤科,全军肿瘤诊治中心,重庆市肿瘤生物技术研究所,重庆,400037 |
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| 摘 要： | 目的研究人0.3 kb的AFP启动子序列对EGFP在人肝癌细胞中表达的影响.方法通过设计含有特定酶切位点的引物,选择性地从人基因组中扩增出人甲胎蛋白启动子(AFP promoter)序列,分别构建含有AFP启动子序列和增强型绿色荧光蛋白(EGFP)及AFP启动子序列和DTA基因的真核表达载体pAF-EGFP、pAF- DTA;将pAF- EGFP转染HepG2、SMMC-7721及NIH3T3后进行荧光强度检测.结果转染pAF-EGFP质粒细胞的EGFP表达量在HepG2细胞中明显高于SMMC-7721、NIH3T3细胞,组间差异显著(P<0.01).结论 AFP启动子可以特异性地启动它后面的目的基因在AFP阳性的细胞中高效表达.
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| 关 键 词： | EGFP AFP启动子 肝癌细胞 |
| 文章编号： | 1000-5404(2004)20-1857-03 |
| 修稿时间： | 2004-07-17 |
Study of AFP promoter on the expression of EGFP in human hepatoma cells |
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| HAO Ping,WANG Dong-lin. Study of AFP promoter on the expression of EGFP in human hepatoma cells[J]. Acta Academiae Medicinae Militaris Tertiae, 2004, 26(20): 1857-1859 |
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| Authors: | HAO Ping WANG Dong-lin |
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| Abstract: | Objective To evaluate the effects of 0.3 kb human alpha-fetoprotein (AFP) promotor on the expression of enhanced green fluorescent protein (EGFP) in human hepatoma cells. Methods By constructing the primer with special enzyme site, human AFP promoter was selectively amplified from human gene bank and eukaryon-expressed pAF-EGFP and pAF-DTA with AFP promotor and EGFP and with AFP promotor and DTA, respectively. pAF-EGFP was transfected into HepG2, SMMC-7721, and NIH3T3. The fluorescence was detected in order to evaluate the transfection rate. Results The expression of EGFP in HepG2 cells transfected with pAF-EGFP was significantly higher than that in SMMC-7721 and NIH3T3 cells (P<0.01). Conclusion AFP promotor can specifically promote and accelerate the expression of target gene within the cell with positive AFP. |
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| Keywords: | EGFP |
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