| 量子点与多肽LyP-1的连接、表征及对肿瘤细胞的识别 |
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| 引用本文： | 潘敏,储茂泉,孙烨,宋馨,祝健,丁祖泉. 量子点与多肽LyP-1的连接、表征及对肿瘤细胞的识别[J]. 生物医学工程学杂志, 2007, 24(3): 577-581 |
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| 作者姓名： | 潘敏 储茂泉 孙烨 宋馨 祝健 丁祖泉 |
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| 作者单位： | 1. 同济大学,生命科学与技术学院,上海,200092
2. 上海交通大学,生命科学与技术学院,上海,200032 |
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| 基金项目： | 国家自然科学基金;上海市纳米科技专项基金 |
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| 摘 要： | 利用水相合成的量子点(Quantum dots,QDs)纳米粒子,通过交联剂[N-succinimidyl3-(2-pyridyldithio)propionate,SPDP]将其与多肽LyP-1(CGNKRTRGC)进行连接以形成一种纳米荧光探针。毛细管电泳、吸收光谱以及荧光光谱测试结果表明,LyP-1已成功地连接到了QDs表面,所得荧光探针能较好地识别SPCA-1肺腺癌细胞,但不识别HL-60原髓细胞白血病成淋巴细胞。
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| 关 键 词： | 量子点 多肽 生物连接 毛细管电泳 光学性质 肿瘤细胞 |
| 修稿时间： | 2005-11-032006-02-20 |
Preparation and Characterization of Quantum dots-peptides Bioconjugations And Their Specificity Related to Recognizing Tumor Cells |
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| Pan Min,Chu Maoquan,Sun Ye,Song Xin,Zhu Jian,Ding Zuquan. Preparation and Characterization of Quantum dots-peptides Bioconjugations And Their Specificity Related to Recognizing Tumor Cells[J]. Journal of biomedical engineering, 2007, 24(3): 577-581 |
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| Authors: | Pan Min Chu Maoquan Sun Ye Song Xin Zhu Jian Ding Zuquan |
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| Affiliation: | 1.School of Life Sicence and Technology, Tongji University, Shanghai 200092, China; 2 .School of Life Sicence and Technology, Shanghai Jiaotong University, Shanghai 200032, China |
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| Abstract: | Water-soluble CdTe quantum dots synthesized in aqueous solution have been conjugated with peptide LyP-1 using N-Succinimidyl 3-[2-pyridyldithio]-propionate(SPDP)as a cross-linking reagent.Capillary electrophoresis(CE),UV-Vis absorption and photoluminescent(PL)spectra suggested that the peptide had been successfully linked to the QDs.The QDs-peptides conjugates could specifically recognize the lung adenoma cancer cells(SPCA-1),but did not recognize promyelocytic leukemia cells(HL-60). |
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| Keywords: | Quantum dots(QDs)Peptide Bioconjugation Capillary electrophoresis(CE)Optical properties Tumor cells |
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