冬凌草抗炎有效部位筛选及初步表征

目的:筛选冬凌草的抗炎有效部位并对有效部位进行初步表征.方法:冬凌草采用梯度极性溶剂水、25%乙醇、50%乙醇、75%乙醇及100%乙醇分别提取,提取物通过蛋清致大鼠足跖肿胀试验以确定抗炎有效部位;并对有效部位采用MS分析,HPLC测定主成分冬凌草甲素的含量.结果:冬凌草100%乙醇提取物对大鼠足跖肿胀有明显的抑制作用,MS分析提取物中明显含有冬凌草甲素及其他贝壳杉烷类二萜,HPLC测定无水乙醇提取冬凌草甲素的提取率为0.174 6%.结论:冬凌草100%乙醇提取物组分具有明显的抗炎作用,为冬凌草的抗炎有效部位,为其临床抗炎提供依据.

Screening and primary characterization of effective anti-inflammatory fractions from Rabdosia rubescens

OBJECTIVE To screen and characterize primarily the effective anti-inflammatory fractions from Rabdosia rubescens.METHODS The plant Rabdosia rubescens was extracted by using water,25% ethanol,50% ethanol,75% ethanol and 100% ethanol.The effective anti-inflammatory fractions were observed by means of egg white inducing rat voix pedis swell model.Then the effective fraction was analyzed by MS and determined by HPLC.RESULTS The 100% ethanol fraction of Rabdosia rubescens had the best anti-inflammatory effects and contained oridonin and analogues.The extraction rate of oridonin in 100% ethanol reached 0.174 6% by HPLC.CONCLUSION The effective fraction for anti-inflammation from Rabdosia rubescens is fraction of 100% ethanol,which provides a reference for clinical application.