胆石六号汤剂对豚鼠胆囊炎治疗作用的观察

目的:研究胆石六号汤剂对盐酸林可霉素致豚鼠胆囊炎的治疗作用.方法:将60只豚鼠按随机数字表法分为6组,即空白对照组,模型组,消炎利胆片组(0.35 g·kg^-1),胆石六号汤剂低剂量组(3.9 g·kg^-1)、中剂量组(7.7 g·kg^-1)、高剂量组(15.4 g·kg^-1),每组10只.除空白组以外,其余各组连续7 d皮下注射盐酸林可霉素建立豚鼠胆囊炎模型,然后灌胃给药30 d.观察豚鼠体质量、胆囊及肝脏质量、胆汁容积、肝脏和胆囊病理组织学改变以及血浆白细胞数量、肝功能变化.结果:胆石六号汤剂组比模型组体质量增加(P <0.01,P <0.05);胆石六号汤剂组能明显减轻模型动物肝湿重和胆囊湿重,改善病理变化,促进胆汁排泄,缓解胆汁淤积(P <0.01,P <0.05);显著降低模型动物血浆白细胞数目(P <0.01);改善肝功能,降低模型动物AST、 ALT、ALP、GGT、TBIL、TBA(P <0.01,P <0.05),且具有一定的剂量依赖性.结论:胆石六号汤剂可以减轻盐酸林可霉素致豚鼠胆囊炎的炎症反应,同时有利胆和保护肝脏的作用.

Observation of therapeutic effects of Danshi Liuhao decoction against cholecystitis in guinea pigs

OBJECTIVE To investigate the therapeutic effects of Danshi Liuhao decoction against cholecystitis in guinea pigs induced by lincomycin hydrochloride.METHODS Sixty guinea pigs were randomly divided into six groups:normal group, model group,positive control group of Xiaoyanlidanpian (0.35 g·kg^-1),low dosage group (3.9 g·kg^-1),middle dosage group (7.7 g·kg^-1) and high dosage group (15.4 g·kg^-1).Each group had 10 animals.Guinea pigs cholecystitis was induced by lincomycin hydrochloride.The animals were treated for 30 days and then the body weight,liver weight,gallbladder weight,gallbladder volume,changes of gallbladder and liver tissues,white blood cell count and hepatic functions were measured.RESULTS Compared with the model group,the Danshi Liuhao decoction resulted in weight gain (P <0.01,P <0.05), obviously decreased wet weights of liver and gallbladder,alleviated pathological changes,promoted biliation and relieved cholestasis (P <0.01,P <0.05), reduced white blood cell count (P <0.01),evidently decreased serum levels of AST,ALT,ALP,GGT,TBIL and TBA (P <0.01,P <0.05),and protected liver in a dose-dependent manner.Animals in Danshi Liuhao decoction groups also had obviously milder inflammation in liver and gallbladder.CONCLUSION Danshi Liuhao decoction has alleviated inflammations,increased bile secretion and protected liver,and it also has beneficial effects on liver and gallbladder.