| Effect of Shenmai Injection on L-type Calcium Current of Diaphragmatic Muscle in Rats |
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| 作者姓名： | 赵丽敏 熊盛道 牛汝楫 徐永健 张珍祥 |
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| 作者单位： | Department of Repiratory Diseases，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Department of Repiratory Diseases，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Department of Repiratory Diseases，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Department of Repiratory Diseases，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Department of Repiratory Diseases，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430030，China，Wuhan 430030，China，Wuhan 430030，China，Wuhan 430030，China，Wuhan 430030，China |
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| 基金项目： | 国家自然科学基金，湖北省教育厅科研项目 |
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| 摘 要： | Diaphragmaticmuscleisanimportantrespiratorymuscle,anddiaphragmaticfatiguecanleadtomanydisorders .Onlyintherecent 2 0 yearshavethesys temicresearchesbeen performedtoinvestigatethefunctionofdiaphragmanditspathophysiology .Di aphragmaticfatiguecanbeinducedby…
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| 关 键 词： | 参脉注射剂 L-型 钙离子通道 肌肉隔膜 老鼠 横膈膜 细胞斑点夹紧技术 |
| 收稿时间： | 2 December 2002 |
Effect of Shenmai Injection on L-type calcium current of diaphragmatic muscle in rats |
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| Zhao?Limin,Xiong?Shengdao,Niu?Ruji,Xu?Yongjian,Zhang?Zhengxiang.Effect of Shenmai Injection on L-type Calcium Current of Diaphragmatic Muscle in Rats[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2004,24(4):376-378. |
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| Authors: | Zhao Limin Xiong Shengdao Niu Ruji Xu Yongjian Zhang Zhengxiang |
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| Institution: | Department of Repiratory Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| Abstract: | Summary In this study, whole cell patch clamp recording technique was employed to investigate the effect of Shenmai Injection (SMI)
on L-type calcium current of diaphragmatic muscle in rats. The result showed that when the diaphragmatic muscle cell was held
at −80 mV and depolarized to +60 mV, 10 μl/ml, 50 μl/ml and 100 μl/ml SMI enhanced the inner peak L-type calcium current from
−(6.8±0.7) pA/pF (n=7) to −(7.3±0.8) pA/pF (P>0.05,n=7), −(8.6±1.0) pA/pF (P<0.05,n=7) and −(9.4±1.2) pA/pF (P<0.05,n=7), respectively. The rates of L-type calcium current were increased by (7.34±2.37) %, (25.72±5.94) %, and (38.16±7.33)%,
respectively. However, it had no significant effect on maximal activation potential and reversal potential. Our results suggested
that SMI could activate the calcium channel of the diaphragmatic fibers of the rats, increase the influx of Ca2+, and enhance the contractility of diaphragmatic muscles.
ZHAO Limin, female, born in 1971, Doctor in Charge
This project was supported by a grant from the National Natural Sciences Foundation of China (No. 39670338) and Program of
Scientific Researches of Hubei Education Bureau (No. 2001C18). |
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| Keywords: | SMI calcium-channel diaphragm patch-clamp techniques |
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