应用荧光原位杂交检测人喉癌中EGFR基因扩增

目的 检测人喉癌Ｈｅｐ－２细胞系和５个喉癌组织中表皮生长因子受体（ｅｐｉｄｅｒｍａｌ ｇｒｏｗｔｈ ｆａｃｔｏｒ ｒｅｃｅｐｔｐｒ，ＥＧＦＲ）基因扩增。方法 荧光原位杂交技术。结果 在Ｈｅｐ－２细胞和２例喉癌组织中期染色体和间期细胞核中，检测到明显的集中成簇和多个斑点分散排布的杂效信号，另３例喉癌组织间期细胞核中杂交信号未见增强或数目增加。结论 以正常二倍休 色体和间期细胞核为对照，在喉癌Ｈｅｐ－

Detection of epidermal growth factor receptor gene amplification in human laryngeal carcinomas by means of fluorescence in situ hybridization

Objective To detect epidermal growth factor receptor (EGFR) gene amplification in human laryngeal carcinoma Hep 2 cell line and laryngeal carcinoma tissues. Methods The technique of fluorescence in situ hybridization(FISH). Results The EGFR gene amplification of a laryngeal carcinoma Hep 2 cell line and 5 laryngeal carcinoma tissues were detected by FISH. In the metaphase chromosome and interphase nuclei of Hep 2 cell line and 2 laryngeal carcinoma tissues, distinct cluster and multiple dot signals were found. In the interphase nuclei of the other 3 laryngeal carcinoma tissues, no increase in the number or extent of the hybrid signals was found. Conclusion Compared with normal diploid cell, the EGFR gene amplification was observed at different levels ranging from 2 to 8 folds in metaphase chromosome and interphase nuclei of Hep 2 cell line and 2 laryngeal carcinoma tissues while no amplification was observed in the other 3 laryngeal carcinoma tissues. The results demonstrate that quantitative detection of amplified gene by FISH in the metaphase chromosome and interphase nuclei is useful for detection of laryngeal carcinoma tissues.