A specific fluorogenic assay for N-acetylgalactosamine-4-sulphatase activity using immunoadsorption |
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| Authors: | D. A. Brooks G. J. Gibson P. A. G. McCourt J. J. Hopwood |
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| Affiliation: | (1) Lysosomal Diseases Research Unit, Department of Chemical Pathology, Adelaide Medical Centre for Women and Children, King William Road, 5006 North Adelaide, South Australia, Australia |
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| Abstract: | Summary A method combining immune capture and enzyme detection by fluorochemistry has been developed for the diagnostic assay of N-acetylgalactosamine-4-sulphatase (4-sulphatase). The procedure uses a monoclonal antibody 4-S 4.1 to immunoadsorb 4-sulphatase specifically from complex protein samples containing other sulphatases, and 4-methylumbelliferyl sulphate to detect captured 4-sulphatase. The assay provides an accurate and simple method for the diagnosis of Maroteaux-Lamy syndrome (Mucopolysaccharidosis type VI). |
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