豚鼠血液及肝、肺组织中钩端螺旋体数量变化与钩体病肺弥漫性出血的关系

作者采用同位素~(32)P标记钩端螺旋体放射性强度测定法和连续倍比稀释培养计数法,动态观察豚鼠血液、肝及肺组织中钩端螺旋体数量的变化,发现后腔静脉和肝中繁殖的钩端螺旋体数量的增加,与肺弥漫性出血的发生密切有关,并与出血的发展在时间和程度上均十分一致。表明在肝中新繁殖并经血液到肺的活钩端螺旋体本身与肺弥漫性出血有直接关系。

RELATIONSHIP BETWEEN THE NUMBER OF LEPTOSPIRES IN BLOOD,LIVERS AND LUNGS OF GUINEA PIGS AND THE PULMONARY DIFFUSE HEMORRHAGE OF LEPTOSPIROSIS

It has been found that there are largenumbers of leptospires in the liver of an in-fected guinea pig, but there are only fewleptospires found in its lungs where thepulmonary diffuse hemorrhage (PDH) takesplace. In order to study the relationshipbetween the two phenomena, we determineddynamically the radioactivity and thenumber of leptospires in the blood, lungs andlivers of the guinea pigs infected by labelled~(32)P and non-labelled leptospires. We have found that the primary leptos-pires with ~(32)P disappeared in the lungs andblood 8-10 hours after the inoculation, butincreased continuously in the liver after theinoculation. According to the results of theculture counting of leptospires, however, theleptospires in the blood and lungs began toincrease constantly twelve hours after theinfection, and the leptospires in the liverincreased from beginning to end. The PDHbegan twelve hours after the inoculation,its development being quite compatiblewith the increase in the number ofleptospires in the liver and blood in respectof time and degree. The increase ofleptospires in the hepatic vein and in theliver coincided very well. Between the num-bers of leptospires in the abdominal aortaand in the hepatic vein, there was nosignificant difference, and the variations ofboth the numbers were parallel. The above results suggest that the filialleptospires were reproduced in the liver,then went continuously into the blood andarrived at the iungs. When flowing throughthe pulmonary capillary vessels, they parti-cipated in the occurrence and developmentof PDH.