| 大麻受体激动剂WIN-55,212-2对肝癌细胞HepG2增殖和凋亡的影响 |
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| 引用本文： | 王映,周于婷,赵青. 大麻受体激动剂WIN-55,212-2对肝癌细胞HepG2增殖和凋亡的影响[J]. 细胞与分子免疫学杂志, 2010, 26(4) |
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| 作者姓名： | 王映 周于婷 赵青 |
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| 作者单位： | 1. 广州医学院生化教研室,广东广州,510182
2. 南方医科大学生物技术学院,广东广州,510515 |
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| 基金项目： | 教育部留学回国人员科研启动基金资助项目(教外司留2007-1108号); 广州市教育局市属高校科技计划项目(61057,08A112); 广州市科技计划项目(2009J1-C361); 广东省中医药局建设中医药强省科研课题(2008362); 广州市留学回国人员科研启动基金(2007-583); 广州医学院留学回国人员启动科研基金(2006GD052) |
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| 摘 要： | 目的:探讨激活大麻受体对肝癌细胞增殖和凋亡的作用,并对其机制进行初步探讨。方法:将细胞分成对照组、不同剂量大麻受体激动剂WIN-55,212-2(WIN)处理组。MTT法测定WIN对HepG2细胞增殖的影响;DNA梯度电泳法检测HepG2细胞凋亡DNA片段,流式细胞术检测分析各组细胞凋亡率变化,Western blot分析各组细胞caspase-3和c-myc的蛋白表达,分光光度法检测caspase-3、8、9的活性。结果:大麻受体激动剂WIN能抑制肝癌细胞生长和c-myc的蛋白表达,诱导肝癌细胞凋亡,上述效应具有剂量依赖性。而且WIN可能通过诱导细胞caspase-3的蛋白表达和激活caspase-3、8、9活性进而诱导肝癌细胞凋亡。结论:大麻受体激动剂WIN能抑制肝癌细胞生长,诱导肝癌细胞凋亡,并与caspases和c-myc的蛋白表达相关。
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| 关 键 词： | WIN-55 212-2 肝癌细胞HepG2 凋亡 增殖 |
The effect of the activation of cannabinoid receptor on the proliferation and apoptosis of hepatoma HepG2 cells |
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| WANG Ying,ZHOU Yu-ting,ZHAO Qing. The effect of the activation of cannabinoid receptor on the proliferation and apoptosis of hepatoma HepG2 cells[J]. Chinese journal of cellular and molecular immunology, 2010, 26(4) |
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| Authors: | WANG Ying ZHOU Yu-ting ZHAO Qing |
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| Affiliation: | WANG Ying1,ZHOU Yu-ting2,ZHAO Qing1 1Department of Biochemistry,Guangzhou Medical College,Guangzhou 510182,2School of Biotechnology,Southern Medical University,Guangzhou 510515,China |
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| Abstract: | AIM:To study the effect of the activation of cannabinoid receptor on the proliferation and apoptosis of hepatoma HepG2 cells. METHODS:HepG2 cells were treated with different doses of the activator of cannabinoid receptor-WIN-55,212-2(WIN),MTT was used to detect the effects of WIN on HepG2 cells; Flow cytometry (FCM) was used to examine the effects of WIN on the apop tosis of HepG2 cells. Caspase 3 and c-myc proteins were determined by Western blot assay,and caspase 3,8,9 activity was measured using a colori... |
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| Keywords: | WIN-55 212-2 hepatoma HepG2 apoptosis proliferation |
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