树突状细胞和巨噬细胞对肝癌细胞抗原的提呈作用

目的 比较同一个体来源的树突状细胞(DC)和巨噬细胞(MΦ)对肝癌细胞抗原的提呈作用。 方法 从同一健康外周血中分离出单核细胞,在粒细胞-巨噬细胞集落刺激因子(GM-CSF)联合白细胞介素-4(IL-4)或单独在GM-CSF的作用下,分别诱导出DC和MΦ;DC或MΦ与自体淋巴细胞和肝癌细胞抗原共培养5d,结束培养前12 h加入37 kBq/孔(1 μci=37 kBq)3H-胸腺嘧啶,收获细胞,用液体闪烁计数仪测定增殖效应(cmp值)。 结果 接触肝癌细胞抗原的DC和MΦ都具有显著地刺激自体淋巴细胞增殖的能力,而以DC的刺激能力更为明显。加20μl/ml抗原时刺激效应最明显,DC组、MΦ组和淋巴细胞组的cpm值分别是11950.3±1621.8、8708.5±176.1和402.5±43.1。结论 同一个体来源的DC的抗原提呈能力明显强于MΦ。

The role of dentritic cell and macrophage in hepatoma antigen-presenting

OBJECTIVE: To study the role of dendritic cells (DCs) and macrophages, differentiated from the same individual peripheral blood monocytes, in tumor antigen- presenting. METHODS: DCs and macrophages were differentiated from human peripheral blood monocytes by adding both Granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) or GM-CSF only. Then they were loaded with tumor antigen at different concentrations and cocultured with autologous T cells in 96-well flat-bottomed microtiter plates for five days at 37 degrees C, 5% CO(2). (3)H-thymine was added before the culture terminated, and twelve hours later, the cells were gathered to test the cpm value. RESULTS: Both DCs and macrophages chased with tumor antigen could strongly stimulate the proliferation of autologous T cells, especially DCs. The stimulation effect with 20 microl/ml antigen was the most remarkable and the cmp values were 11,950.3 +/-1621.8, 8,708.5 +/-176.1, 402.5+/-43.1 in DCs group, Macrophages group, and lymphocytes group, respectively. CONCLUSION: The antigen presenting role of DCs is stronger than that of macrophages from the same individual.