甲状腺激素对新生大鼠脑组织血管内皮细胞生长因子表达的影响

目的 探讨甲状腺激素(TH)对新生大鼠脑血管发育的影响.方法 取18只清洁级SD成年雌性大鼠与3只清洁级SD雄性大鼠交配后随机将孕鼠分入3组:丙基硫氧嘧啶(PTU)干预组、TH干预组和对照组.待每组仔鼠产后第1天即采用电化学发光法检测其血清总三碘甲状腺原氨酸(TT3)、总甲状腺素(TT4)水平;采用酶联免疫吸附试验法测仔鼠脑组织血管内皮细胞生长因子(VEGF)水平.应用SPSS 16.0软件进行统计学分析.结果 TH干预组仔鼠血清TT4(103.50±2.37) μg·L-1]、TT3(1 269.63±32.18) ng·L-1]与脑组织VEGF水平(53.46±10.62) μg·L-1]均高于对照组TT4 (45.11±0.43) μg·L-1,TT3 (844.57± 20.85) ng·L-1,VEGF (11.15±1.33) μg·L-1](Pa<0.001).同时,PTU干预组新生鼠以上各指标TT4 (16.83±0.45) μg·L-1,TT3 (142.35±7.76) ng·L-1,VEGF(8.69±0.48) μg·L-1]均低于对照组(Pa<0.001).结论 TH干预可增加仔鼠脑组织VEGF水平,对脑血管的发育有促进作用.

Effect of Thyroid Hormone on Expression of Vascular Endothelial Growth Factor in Brain of Newborn Rats

Objective To investigate the effect of thyroid hormone(TH) on brain angiogenesis in newborn rats.Methods Eighteen mature virgin females and 3 mature males were selected.Females of each nest were divided into 3 groups randomly after mating:6-N-propyl-2-thiouracil(PTU) regulated group,thyroid hormone(TH) regulated group and control group.Newborns of post neonatal day 1st in each group were used:their serum total thyroxine(TT4),total triiodothyronine(TT3) were estimated by electrochemical luminescence and brain vascular endothelial growth factor(VEGF) were measured by enzyme-linked immunosorbent assay.SPSS 16.0 software was used to analyze the data.Results Serum TT4,TT3,brain VEGF in control groupTT4(45.11±0.43) μg·L-1,TT3(844.57±20.85) ng·L-1,VEGF(11.15±1.33) μg·L-1] were decreased significantly than those in TH regulated group TT4(103.50±2.37) μg·L-1,TT3(1 269.63±32.18) ng·L-1,VEGF(53.46±10.62) μg·L-1](Pa<0.001).However,the levels of serum TT4,TT3 and brain VEGF in PTU regulated group TT4(16.83±0.45) μg·L-1,TT3(142.35±7.76) ng·L-1,VEGF(8.69±0.48) μg·L-1] were decreased significantly compared with those in control group.Conclusions Regulated TH can increase the level of brain VEGF in newborn rats.TH has a substantial impact on vasculature development in the brain of newborn rats.