| 联合应用神经营养素-4胶质细胞源性神经营养因子对脊髓前角运动神经元的保护作用研究 |
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| 引用本文： | 刘刚,潘世鹏. 联合应用神经营养素-4胶质细胞源性神经营养因子对脊髓前角运动神经元的保护作用研究[J]. 实用医技杂志, 2011, 18(8): 795-797 |
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| 作者姓名： | 刘刚 潘世鹏 |
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| 作者单位： | 山西省中铁十七局中心医院,030032 |
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| 摘 要： | 目的研究周围神经损伤后经蛛网膜下腔置管局部联合应用神经营养素（NT）-4和外源性胶质细胞源性神经营养因子（GDNF）对脊髓前角运动神经元的保护作用。方法成年SD大鼠随机分为3组,GDNF组、NT-4组和GDNF联合NT-4组。切断大鼠坐骨神经致相应脊髓前角运动神经元损伤,于第5、6腰椎椎间隙行蛛网膜下腔置管,并注入外源性GDNF（10μg/mL）和NT-4,不同时间处死动物并取材,对L4～6节段脊髓标本冰冻切片,行苏木素-伊红染色、尼氏体染色、胆碱酯酶染色。结果 GDNF联合NT-4组脊髓前角运动神经元的存活率、胆碱酯酶阳性颗粒面积均比前2组有明显提高,组间比较差异有统计学意义（P〈0.05）。结论周围神经损伤后通过蛛网膜下腔联合注入NT-4、GDNF较两者单独使用更有利于保护脊髓运动神经元。
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| 关 键 词： | 周围神经 运动神经元 神经生长因子类 胶质细胞源性神经营养因子 |
Protective effect of glial cell line-derived neurotrophic factor and NT-4 infused into the tube setted into cavitas subarachnoidealis on spinal front corner motor neurons |
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| LIU Gang,PAN Shi-peng. Protective effect of glial cell line-derived neurotrophic factor and NT-4 infused into the tube setted into cavitas subarachnoidealis on spinal front corner motor neurons[J]. Journal of Practical Medical Techniques, 2011, 18(8): 795-797 |
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| Authors: | LIU Gang PAN Shi-peng |
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| Affiliation: | LIU Gang,PAN Shi-peng.The Central Hospital of China Railway No.17 Engineering Bureau,Taiyuan 030032,China |
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| Abstract: | Objective To investigate the effect of exogenous glial cell line-derived neurotrophic factor(GDNF) and NT-4 infused into the cavitas subarachnoidealis on spinal front corner motor neurons after sciatic nerve axotomy.Method Seventy-two healthy SD rats were divided into 3 groups randomly: GDNF group,NT-4 group,GDNF and NT-4 group.The left sciatic nerve in rats were cut off and spinal cord anterior horn motor neurons were damaged.And then NT-4 and GDNF were injected into cavitas subarachnoidealis at L5-6 in gr... |
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| Keywords: | Peripheral nerves Motor neuron Nerve growth factors Glial cell line-derived neurotrophic factor |
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