Rp-cAMPS has ho effect on adenosine A1 receptors in guinea-pig cardiomyocytes |
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| Authors: | Dawn L Adamson Sian E Harding |
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| Institution: | (1) Imperial College of Science, Technology and Medicine, National Heart and Lung Institute, Dovehouse Street, London SW3 6LY, UK, E-mail: d.adamson@ic.ac.uk, GB |
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| Abstract: | Rp-cAMPS, a protein kinase A inhibitor, is used in the investigation of the cAMP-dependent systems. A report by Musgrave et
al. (11) has suggested that Rp-cAMPS may also act on adenosine receptors. To determine whether this occurs in guinea-pig ventricular myocytes, Rp-cAMPS was applied in the presence and absence of DCPCX, an adenosine A1 receptor antagonist. The isoprenaline-induced response was significantly decreased by Rp-cAMPS and the effect was not altered by the presence of DCPCX. Therefore Rp-cAMPS has no effect on cell contraction via adenosine A1 receptors and can reliably be used to investigate cyclic AMP-dependent systems in isolated cardiac myocytes.
Received: 23 April 1999, Returned for 1. revision: 26 May 1999, 1. Revision received: 23 August 1999, Returned for 2. revision:
28 September 1999, 2. Revision received: 15 November 1999, Accepted: 17 November 1999 |
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| Keywords: | Cardiomyocyte – beta-adrenoceptors – Rp-cAMPS – adenosine receptors – proteine kinase A |
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