胸腹水细胞端粒酶活性定性和定量检测

目的探讨端粒酶活性定量检测在诊断良恶性胸腹水中的应用价值。方法采用TRAP-银染定性方法和rrRAP-PicoGreen定量方法，对102例已确诊患者的胸腹水细胞进行端粒酶活性分析。结果恶性胸腹水细胞端粒酶活性明显高于良性胸腹水细胞，其定性检测诊断率明显高于细胞病理学。乳腺癌患者胸腹水细胞的端粒酶活性明显高于卵巢癌、肝癌患者胸腹水细胞的端粒酶活性；肺癌患者胸腹水细胞端粒酶活性明显高于肝癌。在良性胸腹水中，感染性胸腹水细胞端粒酶活性高于非感染性胸腹水。结论恶性胸腹水细胞端粒酶活性明显升高。端粒酶活性定量检测较定性检测更敏感、简便，对良恶性胸腹水的诊断和鉴别诊断有一定应用价值。

Qualitative and quantitative assays for telomerase activity in specimens of ascites/pleural effusion cells

To study the practical feasibility of a quantitative assay for telomerase activity in the diagnosis of malignant and nonmalignant ascites/pleural effusion.The telomerase activity was detected in 102 specimens of ascites/pleural effusion by TRAP-silver staining qualitative and TRAP-PicoGreen quantitative assay respectively. The telomerase activity in malignant ascites/pleural effusion cells was significantly higher than that of nonmalignant ones.The diagnosis rate of qualitative assay was higher than cytopathology.The activity in ascites/pleural effusion cells resulting from breast cancer was higher than that in ovarian and hepatic cancer related;the activity resulting from pulmonary cancer was higher than that in hepatic cancer.The telomerase activity in infective(tuberculosis and pneunonia) ascites/pleural effusion cells was higher than noninfective ones. The telomerase activity in malignant ascites/pleural effusion cells was significantly higher than that in nonmalignant ones.The quantitative assay of telomerase activity is a fast,simple and sensitive than qualitative one as a valuable method in differential diagnosis of malignant and nonmalignant ascites/pleural effusions.