| 三勒浆口服液HPLC指纹图谱的建立及多成分定量测定 |
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| 引用本文： | 姜红,赵生玉,黄浩洲,林俊芝,许润春,张定堃,杨明,杨江林,韩丽.三勒浆口服液HPLC指纹图谱的建立及多成分定量测定[J].中草药,2017,48(18):3741-3747. |
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| 作者姓名： | 姜红 赵生玉 黄浩洲 林俊芝 许润春 张定堃 杨明 杨江林 韩丽 |
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| 作者单位： | 成都中医药大学药学院, 中药材标准化教育部重点实验室, 中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地, 四川 成都 611137;成都中医药大学药学院, 中药材标准化教育部重点实验室, 中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地, 四川 成都 611137;成都中医药大学药学院, 中药材标准化教育部重点实验室, 中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地, 四川 成都 611137;成都中医药大学附属医院 中心实验室, 四川 成都 610072;成都中医药大学药学院, 中药材标准化教育部重点实验室, 中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地, 四川 成都 611137;成都中医药大学药学院, 中药材标准化教育部重点实验室, 中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地, 四川 成都 611137;江西中医药大学, 江西 南昌 330004;成都中医药大学药学院, 中药材标准化教育部重点实验室, 中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地, 四川 成都 611137;成都中医药大学药学院, 中药材标准化教育部重点实验室, 中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地, 四川 成都 611137 |
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| 基金项目： | 国家自然科学基金资助项目（81403115）；国家基础科学人才培养基金（J13100340-19）；成都中医药大学科技发展基金 |
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| 摘 要： | 目的建立三勒浆口服液(SOL)的HPLC指纹图谱,测定其中主要成分量,为SOL的质量控制提供参考。方法采用Welchrom C18色谱柱(250 mm×4.6 mm,5μm),以0.1%磷酸水溶液-甲醇为流动相进行梯度洗脱,体积流量为1.0 m L/min,检测波长270 nm,柱温25℃,建立SOL的HPLC指纹图谱,并对没食子酸、没食子儿茶素、表儿茶素、柯里拉京和鞣花酸指标成分的定量测定进行方法学考察。结果在SOL特征指纹图谱研究中,共确定22个共有峰,同时利用相似度软件对10批SOL指纹图谱进行分析,各批次样品相似度在0.95以上;通过对比特征峰的保留时间,指认出5种主要成分,分别为没食子酸(3号峰)、没食子儿茶素(8号峰)、表儿茶素(15号峰)、柯里拉京(16号峰)和鞣花酸(21号峰);并且10批样品中没食子酸的量在5.743 2~7.538 0 mg/m L,没食子儿茶素的量在0.492 9~0.847 1 mg/m L,表儿茶素的量在0.529 7~0.8048 mg/m L,柯里拉京的量在0.937 5~1.756 5 mg/m L,鞣花酸的量在0.352 7~0.554 5 mg/m L。结论所建立的SOL HPLC指纹图谱和定量测定方法简单,分离效果较好,重复性好,实现了对SOL定性和定量的双重研究,可为其质量控制提供参考。
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| 关 键 词： | 三勒浆 指纹图谱 多组分测定 质量控制 多酚 没食子酸 没食子儿茶素 表儿茶素 柯里拉京 鞣花酸 |
| 收稿时间： | 2017/4/12 0:00:00 |
HPLC fingerprint of Sanlejiang Oral Liquid and content determination of multi-components |
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| JIANG Hong,ZHAO Sheng-yu,HUANG Hao-zhou,LIN Jun-zhi,XU Run-chun,ZHANG Ding-kun,YANG Ming,YANG Jiang-lin and HAN Li.HPLC fingerprint of Sanlejiang Oral Liquid and content determination of multi-components[J].Chinese Traditional and Herbal Drugs,2017,48(18):3741-3747. |
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| Authors: | JIANG Hong ZHAO Sheng-yu HUANG Hao-zhou LIN Jun-zhi XU Run-chun ZHANG Ding-kun YANG Ming YANG Jiang-lin and HAN Li |
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| Institution: | Pharmacy College, Chengdu University of Traditional Chinese Medicine;Key Laboratory Breeding Base of Systematic Research and Utilization on Chinese Material Medical Resources Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China;Pharmacy College, Chengdu University of Traditional Chinese Medicine;Key Laboratory Breeding Base of Systematic Research and Utilization on Chinese Material Medical Resources Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China;Pharmacy College, Chengdu University of Traditional Chinese Medicine;Key Laboratory Breeding Base of Systematic Research and Utilization on Chinese Material Medical Resources Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China;Affiliated Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu 610072, China;Pharmacy College, Chengdu University of Traditional Chinese Medicine;Key Laboratory Breeding Base of Systematic Research and Utilization on Chinese Material Medical Resources Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China;Pharmacy College, Chengdu University of Traditional Chinese Medicine;Key Laboratory Breeding Base of Systematic Research and Utilization on Chinese Material Medical Resources Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China;Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China;Pharmacy College, Chengdu University of Traditional Chinese Medicine;Key Laboratory Breeding Base of Systematic Research and Utilization on Chinese Material Medical Resources Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China;Pharmacy College, Chengdu University of Traditional Chinese Medicine;Key Laboratory Breeding Base of Systematic Research and Utilization on Chinese Material Medical Resources Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China |
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| Abstract: | |
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| Keywords: | Sanlejiang fingerprint multi-components determination quality control polyphenols gallic acid gallocatechin epicatechin corilagin ellagic acid |
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