电针心经不同节段对家兔心上交感神经丛放电活动的影响

背景:经脉脏腑相关是针灸经络理论的核心,也是针灸作用机制研究的切入点和突破口。本文从心经与支配心脏交感神经电活动关系的角度探讨针灸经络理论的核心内容。目的:观察电针心经不同节段对家兔心上交感神经丛放电活动的影响。设计:随机对照动物实验。单位:安徽中医学院经脉脏腑相关研究中心实验室。材料:实验于2004-03在安徽中医学院经脉脏腑相关研究中心实验室开展,共选健康青紫兰家兔36只,体质量(2.8±0.3)kg。随机分为3组,每组12只,即电排针刺激心经腕部(“神门”区)、肘部(“少海”区)和腋部(“极泉”区)经脉主干3组。方法:每次每组各选1只同性别、体质量差小于10%的家兔,同步进行实验。在0lympus解剖显微镜(日本)下分离心上交感神经丛,埋植多股不锈钢丝双极电极,进口专用凝胶(美国产)固定后,关闭胸腔,控制肛温(39±0.5)℃,50mL/L葡萄糖等渗液静脉滴注维护2h后电针。记录分析心率变异性和心上交感神经丛放电活动的变化。主要观察指标:电针后各组动物的心率变异性、功率谱高频/低频值分析。结果:纳入家兔共3组,每组12只,均进入结果分析。电针后,各组心率变异性的总心率变异性值和心上交感神经丛放电活动频率增高,功率谱高频/低频比值下降,以电针腕部组为著,肘部组次之,腋部组最弱。结论:电针心经不同节段,均可促进心上交感神经丛放电活动,增强左心功能,且以心经腕部经脉主干与左心功能间关系最为密切。

Effect of electroacupuncture at different segments of heart meridian on the electric discharge activities of the superior sympathetic plexus in rabbits

BACKGROUND: Correlation between channel and viscera is a core of a themry on acupuncture and meridian, and is also a key entrance for mech anism of acupuncture. This study was designed to discuss theory on acupuncture and meridian through correlation between heart meridian and electric discharge activities of sympathetic nerve. OBJECTIVE: To investigate the effect of electroacupuncture at different segments of heart meridian on the electric discharge activities of the supe rior sympathetic plexus in rabbits. DESIGN: Randomized controlled animal study. SETTING: Central Laboratory of Channel and Viscera Research, Anhui College of Traditional Chinese Medicine. MATERIALS: The experiment was carried out at the Central Laboratory of Channel and Viscera Research, Anhui College of Traditional Chinese Medicine in March 2004. A total of 36 healthy chinchilla rabbits weighing (2.8±0.3) kg were randomly divided into three groups with 12 in each group, including multi-electro-acupuncturing the wrist (Shenmen region), elbow (Shaohai region) or axils (Jiquan region) sections of heart meridian. METHODS: One rabbit of the same sex whose body mass was less than 10% was selected from each group. Superior sympathetic plexus was sepa rated under Olympus anatomic microscope (Japan), and multiple double electrodes materialized as stainless steel sil were embedded. Then, after fixing with special gelatum (USA), thoracic cavity was closed, temperature in anus was controlled at (39±0.5) ℃, and 50 mL/L glucose was dripped into vein for 2 hours before acupuncture. The changes of heart rhythm vari ability (HRV) and electric discharge activities of superior sympathetic plexus were recorded and analyzed. MAIN OUTCOME MEASURES: HRV, ratio between high frequency (HF) and low frequency (LF) of power spectrum. RESULTS: All rabbits were involved in the final analysis. After elec troacupuncture, the total HRV values and electroactivitic frequencies of the superior sympathetic plexus were increased, but LF/HF of power spectrum was decreased. The decrease at the wrist section was superior to others; the axils section was inferior to elbow section. CONCLUSION: Electroacupuncture at different segments of heart meridi an can improve the electric discharge activities of the superior sympathetic plexus and the leaf-cardiac function. Among the three groups, the relation ship of functions was the closest between the wrist stem of heart meridian and left heart.