提取工艺对半夏急性毒性与相关物质基础含量的影响研究

目的考察提取工艺对半夏相关毒性物质基础含量与急性毒性的影响。方法运用UV法、HPLC法、酸碱滴定法和经典急性毒性实验法,进行半夏不同提取工艺样品的含量测定和急性毒性比较研究。结果半夏不同提取工艺样品总有机酸含量：75%醇渗液〉75%醇提液〉30%醇提液〉30%醇渗液〉水提组分≈水渗液;总生物碱含量：75%醇渗液〉75%醇提液〉30%醇渗液〉30%醇提液〉水渗液≈水提组分。毒性研究结果显示：75%醇渗液和75%醇提液可测出MTD值,其余组测得MLD值,毒性大小依次为75%醇渗液〉75%醇提液〉30%醇渗漉组〉30%醇提组〉水渗组〉水提组。结论提取工艺对半夏毒性及相关毒性物质含量有一定的影响,其毒性大小与总生物碱、总有机酸含量呈一定相关性。

Experimental Research on the Influences of Extracting Process on the Content of Toxical Substances and Acute Toxicity of Rhizoma Pinelliae

Objective To compare the content of toxical substances and the acute toxicity of Rhizoma Pinelliae in different processing,and evaluate the processing＇s influences on the content of toxical substances and acute toxicity.Methods UV method,HPLC method,acid-base titration and the classical method of acute toxicity were used to determine the content of toxical substances and compare the acute toxicity of Rhizoma Pinelliae in different processing.Results The content of total organic acid is： 75% alcohol-infiltration75% alcohol-extraction30%alcohol-extraction 30% alcohol-infiltrationwater-infiltration≈water-extraction;the content of total alkaloid is： 75% alcohol-infiltration 75% alcohol-extraction30% alcohol-infiltration 30% alcohol-extraction water-infiltration ≈water-extraction.MTD value can betested in 75% alcohol-infiltration and 75% alcohol-extraction groups,while MLD value can be tested in other groups.The comparation of acute toxicity is： 75% alcohol-infiltration75% alcohol-extraction30% alcohol-infiltration30% alcohol-extractionwater-infiltrationwater-extraction.Conclusion Extraction has certain influence on the content of toxical substances and acute toxicity of Rhizoma Pinelliae,and the toxicity of Rhizoma Pinelliae has some relevance with the content of total organic acid and total alkaloid.