| Abstract: | Objective: To screen and identify the mimotopes of lipopolysaccharide (LPS) epitope. Methods: A random phage display dodecapeptide library was screened with monoclonal antibody 2B4 specifically against LPS conservative epitope. The positive clones were identified by phage EUSA and competitive inhibition assay with either S. typhimurium T861 LPS or E. coli Olll:B4 LPS. Results: After 3 rounds of biopanning, the clones bound with monoclonal antibody 2B4 were well enriched with the positive rate of 80%. The bindings between 12 positive phage clones and screening antibody were inhibited by both kinds of LPS. The positive peptide sequences were deduced from the corresponding DNA sequences. There were some identical sequences among them: PPQWFFSQPQL (5/12, 41. 7%), LPQYFW NTATTA (3/12, 25%), FPQNHWNVPWAT(2/12, 16. 6% ),HSQSFWNAPLAM and AHPWTHGYFPPL (l/12, 8. 3% ). Conclusion: The peptides screened with 2B4 antibody are mimotopes of LPS conservative epitope. |
