| Study of renaturation condition of anti-TNF-α recombinant antibodies |
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| 引用本文： | 陈萍,韩骅,蒲勤,邓健蓓,药立波,苏成芝.Study of renaturation condition of anti-TNF-α recombinant antibodies[J].中国人民解放军军医大学学报,2000(2). |
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| 作者姓名： | 陈萍 韩骅 蒲勤 邓健蓓 药立波 苏成芝 |
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| 作者单位： | Chen Ping; Han Hua; Pu Qing; Deng Jianbei; Yao Libo; Su Chengzhi Department of Biochemistry and Molecular Biology，Fourth Military Medical University，Xi' an，710033 |
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| 基金项目： | Supported by National Natural Science Foundation of China, No. 39670695 |
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| 摘 要： | Recolnbinant Protein of antibody empressed in foreignhosts (generaly Eschewhia colt) may accumulate in insoluble fonnS--inclusion bodies, which has not any biological activity. In most cases, insoluble inclusion bodiestend to Predondnate at higher expression levels. Recentstudies have revealed that the refolding of protein as wellas dissolving inclusion bodies into functional Protein mayl)e associated with denatu~ and renatllrant. In this paperl we used two eXPression productS as objects, w…
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