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Expression of the gene for the atrial natriuretic peptide in cardiac myocytes in vitro |
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| Authors: | David G. Gardner Jiangping Wu Margot C. LaPointe Brenda K. Hedges Christian F. Deschepper |
| Affiliation: | (1) Metabolic Research Unit, University of California at San Francisco, San Francisco;(2) the Department of Medicine, University of California at San Francisco, San Francisco;(3) the Department of Physiology, University of California at San Francisco, San Francisco;(4) Metabolic Research Unit, University of California, 94143 San Francisco, California;(5) the Department of Medicine, University of California, 94143 San Francisco, California;(6) the Department of Physiology, University of Californai, 94143 San Francsco, California |
| Abstract: | Summary Primary cultures of neonatal cardiocytes expreses the gene for atrial natriuretic peptide (ANP). In general the levels of expression follow the rank order: atrial > ventricular  nonmyocardial cells. Following the initial dispersion cardlocytes require 48 to 72 hours before ANP seeretion and ANP mRNA accumulation approach a new steadystate level. In situ hybridization analysis indicates that ANP gene expression is concentrated in a subpopulation of cardiocytes in both the atrial and the ventricular cell cultures. These findings suggest that these primary cultures may be of value in defining the faciors governing the expression of the ANP gene in the cardiac cell. |
| Keywords: | Atrial natriuretic peptide cardiac myocytes gene expression hybridization |
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