射频消融治疗术对兔肝脏肿瘤影响的实验研究及意义初探

目的 :探讨射频消融对肿瘤组织的治疗效果及其意义 .方法 :通过外科手术将VX2肿瘤组织混悬液穿刺注射建立新西兰兔肝脏肿瘤模型 ,按照射频治疗的先后顺序将模型动物随机分为射频治疗前 (对照 )、射频治疗后 1 ,3,5 ,7和 1 4d 6个时间组 ,分别对其肝功能、肿瘤B超影像学及病理学改变进行观察 .结果 :1wk内肝功能出现明显异常改变 ,多数指标在 2wk左右恢复 (P <0 .0 5 ) ;射频治疗 1wk内B超影像学提示肿瘤大小无明显变化 ,之后逐渐缩小 ;RF治疗后肿瘤组织会形成坏死区、损伤区和炎性区 .随着时间的推移 ,部分坏死区会形成液化区 ,部分损伤区会出现坏死 .细胞凋亡大量存在于治疗区内 .结论 :射频消融治疗术对肝脏肿瘤疗效确切 ,细胞凋亡可能是射频消融治疗肝脏肿瘤的一项重要机制

Treatment of rabbit hepatic neoplasm with radiofrequency ablation

AIM: To analyze the therapeutic effect and significances of radiofrequency ablation (RFA) on the hepatic neoplasm. METHODS: The hepatic neoplasm animal model was established by injecting the VX2 tumor constitution mass into the liver of the rabbits. The animal models were randomly divided into the control group (before RF), and 0, 1, 3, 5,7 and 14 d groups after RF, and then the changes of hepatic function, hepatic neoplasm ultrasonography and pathology were observed respectively. RESULTS: Hepatic function changed apparently in 1 week and restored gradually to the normal level in 2 week ( P < 0.05) . The size of neoplasm changed little in 1 week after RF and then reduced gradually. The neoplasm tissue formed necrosis, injury and inflammation regions after RF. Some of necrosis regions liquefied, and some of injury regions formed necrosis. Cell apoptosis occurred in the therapeutic region widely. CONCLUSION: The therapeutic effect of RF on the hepatic neoplasm is positive. Cell apoptosis is one of the key mechanism of RF treatment of the hepatic neoplasm.