制备壳聚糖微球体外缓释TGF-β_1的研究

应用离子交联沉淀法制备壳聚糖-转化生长因子（TGF-β1）缓释微球,研究其体外缓释性能。采用离子交联沉淀法制备壳聚糖微球,以其包裹TGF-β1,制备具有缓释效能的壳聚糖-TGF-β1缓释微球。用扫描电镜、激光粒度分析仪、Elisa法等观察其表面形态,测定药物载药率、包封率、体外缓释效率等指标。结果表明：所得微球球形良好,表面光滑,粒径分布集中,平均粒径272nm。壳聚糖微球有较高的药物包封率,达80.60%。体外释放试验提示,TGF-β1初期存在突释现象,前24h释放达27%,但其后可从壳聚糖微球中稳定释放,7d累计释放达41%。离子交联沉淀法制备壳聚糖缓释微球方法简单易行,所得壳聚糖-TGF-β1微球具有良好的缓释效能,提示其在组织工程领域具有良好的应用前景。

In Vitro Study of TGF- β1 Loaded Chitosan Microspheres

To prepare transformiog growth factor β1 （TGF- β1 ） loaded chitosan microspheres and study the properties of controlled release of TGF - β1 in vitro. We prepared chitosan microspheres with ionically cross - hnkiog precipitant method, and then packaged TGF - β1 in the micmspheres. The features such as the size distribution, drug content and in vitro release were studied. Results showed that the micmspheres were spherical and smooth. The mean diameters of the spheres were 272 nm, and the higher encapsulation efficiency was obtained （about 80.6% ）. The sudden release of TGF - β1 was observed in the initial period in test of release in vitro, and then TGF - β1 could be constantly released from the chitosan microspheres （up to 41% in 7 days）. The method preparing chitosan microspheres with precipitant is convenient and feasible, and TGF- β can be sustained released, which implies potential use in tissue engineering field.