| HPLC同时测定苦豆子中槐定碱、氧化槐果碱和氧化苦参碱的含量 |
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| 引用本文： | 古丽娜·沙比尔,阿吉艾克拜尔·艾萨,石明辉,贾晓光. HPLC同时测定苦豆子中槐定碱、氧化槐果碱和氧化苦参碱的含量[J]. 中国中药杂志, 2007, 32(24): 2619-2622 |
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| 作者姓名： | 古丽娜·沙比尔 阿吉艾克拜尔·艾萨 石明辉 贾晓光 |
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| 作者单位： | 1. 新疆中药民族药研究所,新疆,乌鲁木齐,830002
2. 中国科学院,新疆理化技术研究所,新疆,乌鲁木齐,830011 |
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| 基金项目： | 新疆维吾尔自治区少数民族科技人才特殊培养专项基金(2005-6) |
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| 摘 要： | 目的：建立同时测定苦豆子中槐定碱、氧化槐果碱及氧化苦参碱含量的HPLC测定法。方法：色谱柱为Phenomenex Gemini C18(46 mm×250 mm,5 μm),以甲醇(A)-02%磷酸水(B)为流动相,检测波长205 nm,流速1 mL·min-1,柱温30 ℃。结果：在线性范围内3个对照品的标准曲线呈良好的线性关系(r≥0999 7)。平均回收率分别为1000%,970%,970%。结论：可通过HPLC同时测定苦豆子中槐定碱、氧化槐果碱和氧化苦参碱的含量,并可用该方法进行苦豆子药材的质量控制。
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| 关 键 词： | 苦豆子 HPLC法 槐定碱 氧化槐果碱 氧化苦参碱 |
| 文章编号： | 1001-5302（2007）24-2619-04 |
| 收稿时间： | 2007-03-01 |
| 修稿时间： | 2007-03-01 |
Simultaneous determination of contents of sophoridine, oxymatrine and oxysophocarpine in seed of Sophora alopecuroides by HPLC |
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| Gulnar Sabir; Haji Akber Aisa; Shi Ming-hui; JIA Xiao-guang. Simultaneous determination of contents of sophoridine, oxymatrine and oxysophocarpine in seed of Sophora alopecuroides by HPLC[J]. China Journal of Chinese Materia Medica, 2007, 32(24): 2619-2622 |
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| Authors: | Gulnar Sabir Haji Akber Aisa Shi Ming-hui JIA Xiao-guang |
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| Affiliation: | Xingjiang Institute of Chinese Material Medica and Ethica, Urumch 830002, China. gulnar_sabir@163.com |
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| Abstract: | OBJECTIVE: To develop a HPLC method for simultaneous determination of the contents of sophoridine, oxymatrine and oxysophocarpine in seed of Sophora alopecuroides. METHOD: Chromatographic condition included a Phenomenex Gemini C18 (4.6 mm x 250 mm, 5 microm) column and thmobile phase consisting of a mixture of methanol-0.2% phosphatic acid (7:93). The detection wavelength was at 205 nm. The flow rate was 1.0 mL x min(-1) and column temperature was set at 30 degrees C. RESULT: All calibration curves showed good linearity (r > or = 0.9997) within test ranges. The mean recoveries were 100.0%, 97.0% and 97.0% respectively. CONCLUSION: This method is simple, rapid and accurate, and suitable for quality control of S. alopecuroides seed. |
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| Keywords: | Sophora alopecuroides seed HPLC sophoridine oxysophocarpine oxymatrine |
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